Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	383,004	T→C	L52L (CTA→CTG)	yaiP ←	predicted glucosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	383,004	0	T	C	100.0%	78.4 / NA	29	L52L (CTA→CTG)	yaiP	predicted glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/29);  total (0/29)

CGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGT  >  W3110S.gb/382981‑383041                        |                                      cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:1134430/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:994662/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:961699/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:91127/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:893071/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:820849/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:803047/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:78959/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:673149/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:66391/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:645283/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:637059/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:569104/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:522030/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:499259/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:408882/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:355229/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:285733/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:163243/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:140789/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:1393210/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:1370263/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:1344927/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:1338350/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:126873/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:1266592/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:1261895/61‑1 (MQ=255) cGGCAAAAATAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:1006209/61‑1 (MQ=255)         aTAAGGGTTACGCAGCAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGt  <  1:998682/53‑1 (MQ=255)                        |                                      CGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGT  >  W3110S.gb/382981‑383041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑