Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,771,136	G→A	intergenic (‑96/‑5)	cbrA ← / → yidR	predicted oxidoreductase, FAD/NAD(P)‑binding domain/conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,771,136	0	G	A	100.0%	103.4 / NA	39	intergenic (‑96/‑5)	cbrA/yidR	predicted oxidoreductase, FAD/NAD(P)‑binding domain/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/39);  total (0/39)

CCTTGTGAAGTATGATGGCGGGACCCGTTTTGTATCAGGACCGCGCCATGAAACAGATAACC  >  W3110S.gb/3771094‑3771155                                           |                    ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:61769/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1014532/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:469729/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:486456/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:494532/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:539872/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:55125/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:560005/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:572792/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:446594/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:632356/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:63541/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:666293/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:675512/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:683418/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:710907/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:8200/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:926181/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1187236/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1023760/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1061197/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1072758/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1103659/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1110586/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1131566/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1154246/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1161981/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:265683/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1288589/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1314429/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1326944/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:13301/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1364470/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1376651/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1411874/62‑1 (MQ=255) ccTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:1447657/62‑1 (MQ=255)  cTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:735513/61‑1 (MQ=255)  cTTGTGAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:837046/61‑1 (MQ=255)      tgAAGTATGATGGCGGGAACCGTTTTGTATCAGGACCACGCCATGAAACAGATAAcc  <  1:476976/57‑1 (MQ=255)                                           |                    CCTTGTGAAGTATGATGGCGGGACCCGTTTTGTATCAGGACCGCGCCATGAAACAGATAACC  >  W3110S.gb/3771094‑3771155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑