Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,798,173	T→C	A53A (GCT→GCC)	yicN →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,798,173	0	T	C	100.0%	37.0 / NA	15	A53A (GCT→GCC)	yicN	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/15);  total (0/15)

GGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGATGAGTCGCACCTGCA  >  W3110S.gb/3798162‑3798223            |                                                   ggAAAGTCAGCCGGTGACGAATTTTTACGTTATTTGCATCGCCCGGATTAGTCGCACCTGCa  <  1:887810/62‑1 (MQ=255) ggAAAGTCAGCCGGTGACGAATTTTTACGTTATTTGCATCGCCCGGATGAGTCGCACCTGCa  <  1:1025849/62‑1 (MQ=255) ggAAAGTCAGCCGGTGACGAATTTTTACGTTATTTGCATCGCCCGGATGAGTCGCACCTGCa  <  1:1148692/62‑1 (MQ=255) ggAAAGTCAGCCGGTGACGAATTTTTACGTTATTTGCATCGCCCGGATGAGTCGCACCTGCa  <  1:1152426/62‑1 (MQ=255) ggAAAGTCAGCCGGTGACGAATTTTTACGTTATTTGCATCGCCCGGATGAGTCGCACCTGCa  <  1:1174095/62‑1 (MQ=255) ggAAAGTCAGCCGGTGACGAATTTTTACGTTATTTGCATCGCCCGGATGAGTCGCACCTGCa  <  1:1204081/62‑1 (MQ=255) ggAAAGTCAGCCGGTGACGAATTTTTACGTTATTTGCATCGCCCGGATGAGTCGCACCTGCa  <  1:314286/62‑1 (MQ=255) ggAAAGTCAGCCGGTGACGAATTTTTACGTTATTTGCATCGCCCGGATGAGTCGCACCTGCa  <  1:429520/62‑1 (MQ=255) ggAAAGTCAGCCGGTGACGAATTTTTACGTTATTTGCATCGCCCGGATGAGTCGCACCTGCa  <  1:501052/62‑1 (MQ=255) ggAAAGTCAGCCGGTGACGAATTTTTACGTTATTTGCATCGCCCGGATGAGTCGCACCTGCa  <  1:560914/62‑1 (MQ=255) ggAAAGTCAGCCGGTGACGAATTTTTACGTTATTTGCATCGCCCGGATGAGTCGCACCTGCa  <  1:817531/62‑1 (MQ=255) ggAAAGTCAGCCGGTGACGAATTTTTACGTTATTTGCATCGCCCGGATGAGTCGCACCTGCa  <  1:888537/62‑1 (MQ=255) ggAAAGTCAGCCGGTGACGAATTTTTACGTTATTTGCATCGCCCGGATGAGTCGCACCTGCa  <  1:99955/62‑1 (MQ=255)  gAAAGTCAGCCGGTGACGAATTTTTACGTTATTTGCATCGCCCGGATGAGTCGCACCTGCa  <  1:1127236/61‑1 (MQ=255)        cAGCCGGTGACGAATTTTTACGTTATTTGCATCGCCCGGATGAGTCGCACCTGCa  <  1:440688/55‑1 (MQ=255)            |                                                   GGAAAGTCAGCTGGTGACGAATTTTTACGTTATTTGCATCGTCCGGATGAGTCGCACCTGCA  >  W3110S.gb/3798162‑3798223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑