| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,800,283 | C→G | intergenic (‑81/‑141) | yicS ← / → nlpA | hypothetical protein/cytoplasmic membrane lipoprotein‑28 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,800,283 | 0 | C | G | 95.6% | 246.2 / 1.6 | 91 | intergenic (‑81/‑141) | yicS/nlpA | hypothetical protein/cytoplasmic membrane lipoprotein‑28 |
| Reads supporting (aligned to +/- strand): ref base C (4/0); new base G (0/87); total (4/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.74e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01 | |||||||||||
AATGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTT > W3110S.gb/3800231‑3800316 | aaTGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCtt > 1:659581/1‑59 (MQ=255)aaTGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCg > 1:77760/1‑62 (MQ=255)aaTGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCg > 1:562409/1‑62 (MQ=255)aaTGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCg > 1:560949/1‑62 (MQ=255) tGCTGTACGAGAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:281007/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCATACtt < 1:941028/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:531575/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:67098/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:632528/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:629398/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:624727/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:599508/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:595177/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:587672/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:692424/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:509055/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:45596/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:451451/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:439848/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:432004/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:430476/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:429971/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:41809/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:395545/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:855852/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:981305/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:972941/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:971595/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:971479/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:944827/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:943309/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:927976/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:914339/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:913543/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:910358/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:681604/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:852753/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:818671/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:790288/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:767915/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:760396/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:747186/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:740015/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:738065/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:381533/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1211702/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1000157/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:127185/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1248613/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1243753/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1240845/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1230751/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1224231/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1223381/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1216933/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1216837/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1288699/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1209023/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:120596/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1199559/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1185869/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1178005/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1139231/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1094475/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1089118/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1053101/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1014626/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:256147/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:369669/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:368037/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:346203/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:344880/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:325275/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:321015/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:311975/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:310036/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:257343/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:256544/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1349292/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:182174/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:171320/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:165702/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:151140/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:148917/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1465535/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1449996/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1420149/46‑1 (MQ=255) tGCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:1394551/46‑1 (MQ=255) gCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:247263/45‑1 (MQ=255) gCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:854551/45‑1 (MQ=255) gCTGTACGAAAGGTGTTTGCGTGGTCAGTAAGAAGTGCCAGACtt < 1:937122/45‑1 (MQ=255) | AATGAAGCATAAGTGTAGTTTGCTTTCATCGCTCTCATCATGCTGTACGAAACGCGCTTGCGTGGTCAGTAAGAAGTGCCAGACTT > W3110S.gb/3800231‑3800316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |