Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,862,182 A→G D279D (GAT→GAC)  lldP ← L‑lactate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,862,1820AG100.0% 343.3 / NA 119D279D (GAT→GAC) lldPL‑lactate permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (119/0);  total (119/0)

Only 100 of 119 total aligned reads displayed.

CTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAGGAACA  >  W3110S.gb/3862167‑3862228
               |                                              
gtgtgACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:313371/2‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTg                   >  1:310737/1‑45 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGt            >  1:1267857/1‑52 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGaa    >  1:1385916/1‑60 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAAc   >  1:535381/1‑61 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAAc   >  1:1254814/1‑61 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAAc   >  1:318957/1‑61 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAAc   >  1:885319/1‑61 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:588646/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:50889/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:594026/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:617109/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:620198/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:625830/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:628879/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:636707/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:666176/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:583395/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:693940/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:500096/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:465663/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:457933/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:420790/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:405083/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:404377/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:374817/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:374421/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:348288/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:817155/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:990843/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:9740/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:9561/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:951616/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:947038/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:915572/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:907105/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:865585/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:864359/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:825262/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:817871/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:692808/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:796530/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:791863/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:771154/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:761514/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:759517/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:756545/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:754289/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:743913/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:705757/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:703914/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:339072/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1156443/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1341714/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1322184/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1306166/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1293709/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:127801/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1273010/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1237004/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1209842/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1197932/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1181954/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1167293/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1345015/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1144114/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1099974/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1099387/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:109243/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1085690/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1076777/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1071650/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1060604/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1053077/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:105028/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1025563/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:223908/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1016004/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:336180/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:302162/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:280914/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:278083/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:262982/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:245119/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:242138/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:235311/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:340160/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:212206/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:195654/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:191687/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1461238/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1402958/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1401841/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1384319/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1374748/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:1371450/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:135095/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGATTGAGGAACa  >  1:498600/1‑62 (MQ=255)
cTGTGACGCCCCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGAGTTTGAGGAAc   >  1:580227/1‑61 (MQ=255)
cTGTGACGCACCTAAGTCACCGAAGCGGAATACGCGCACTGGTTGCCAGCGTTTGAGGAACa  >  1:498636/1‑62 (MQ=255)
               |                                              
CTGTGACGCCCCCAAATCACCAAAACGGAATACACGCACTGGCTGCCAGCGTTTGAGGAACA  >  W3110S.gb/3862167‑3862228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑