| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,909,227 | A→C | A20A (GCT→GCG) | xylF ← | D‑xylose transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,909,227 | 0 | A | C | 88.8% | 438.6 / 32.6 | 169 | A20A (GCT→GCG) | xylF | D‑xylose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (19/0); new base C (0/150); total (19/150) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.63e-25 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01 | |||||||||||
Only 100 of 169 total aligned reads displayed.
GCCATACCTATTTTGACTTCTTTGGCGTGTGCAGCAACGTTGGTAAGCAGGAGTGAGGTG > W3110S.gb/3909195‑3909254 | gCCATACCTATTTTGACTTCTTTGGCGTGTGCAGCAACGTTGGTAAGCAGGAGTGAGGt > 1:10566/1‑59 (MQ=255)gCCATACCTATTTTGACTTCTTTGGCGTGTGCAGCAACGTTGGTAAGCAGGAGTGAGGTg > 1:1348791/1‑60 (MQ=255)gCCATACCTATTTTGACTTCTTTGGCGTGTGCAGCAACGTTGGTAAGCAGGAGTGAGGTg > 1:791136/1‑60 (MQ=255)gCCATACCTATTTTGACTTCTTTGGCGTGTGCAGCAACGTTGGTAAGCAGGAGTGAGGTg > 1:518523/1‑60 (MQ=255)gCCATACCTATTTTGACTTCTTTGGCGTGTGCAGCAACGTTGGTAAGCAGGAGTGAGGTg > 1:798979/1‑60 (MQ=255)gCCATACCTATTTTGACTTCTTTGGCGTGTGCAGCAACGTTGGTAAGCAGGAGTGAGGTg > 1:167032/1‑60 (MQ=255)gCCATACCTATTTTGACTTCTTTGGCGTGTGCAGCAACGTTGGTAAGCAGGAGTGAGGTg > 1:1307770/1‑60 (MQ=255)gCCATACCTATTTTGACTTCTTTGGCGTGTGCAGCAACGTTGGTAAGCAGGAGTGAGGTg > 1:866190/1‑60 (MQ=255)gCCATACCTATTTTGACTTCTTTGGCGTGTGCAGCAACGTTGGTAAGCAGGAGTGAGGTg > 1:299783/1‑60 (MQ=255)gCCATACCTATTTTGACTTCTTTGGCGTGTGCAGCAACCTTGGTAAGCAGGAGTGAGGTg > 1:817482/1‑60 (MQ=255)gCCATACCTATTTTGACTTCTTTGGAGTGTGCAGCAACGTTGGTAAGCAGGAGTGAGGTg > 1:942150/1‑60 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTTGTAAGCa < 1:286851/39‑1 (MQ=38) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGcg < 1:172952/39‑2 (MQ=39) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:540438/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:539761/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:589365/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:525753/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:577428/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:583142/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:678542/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:522267/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:487543/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:478716/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:467696/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:454574/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:452989/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:43393/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:413055/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:359598/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:344852/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:341897/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:846231/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1000739/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:971902/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:954070/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:932193/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:918298/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:892563/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:875123/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:870286/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:868838/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:8485/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:638163/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:808102/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:797194/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:778837/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:757341/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:727814/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:688341/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:982386/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:661967/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:652917/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:650962/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1143535/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:135556/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:132353/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1304065/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1262471/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1260105/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1259819/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1224252/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1204761/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1195383/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1190476/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:114874/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1386748/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:114194/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1120749/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1084948/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1084348/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:106839/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1060733/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1051340/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1036771/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1034751/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1021693/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1017661/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:176672/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:31117/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:25345/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:253232/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:242058/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:227042/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:226579/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:214186/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:199956/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:192793/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:186951/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:185067/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:333742/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:154944/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:152069/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1472640/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1452897/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1450054/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:144281/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1420354/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1415748/39‑1 (MQ=255) ttttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:1398821/39‑1 (MQ=255) ttGACTTCTTTGGCGTGTGCCGCAACGTTGGTAAGCa < 1:553129/37‑1 (MQ=255) | GCCATACCTATTTTGACTTCTTTGGCGTGTGCAGCAACGTTGGTAAGCAGGAGTGAGGTG > W3110S.gb/3909195‑3909254 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |