Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,915,241	A→G	E48G (GAG→GGG)	glyQ →	glycine tRNA synthetase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,915,241	0	A	G	100.0%	75.7 / NA	28	E48G (GAG→GGG)	glyQ	glycine tRNA synthetase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/0);  total (28/0)

TGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCC  >  W3110S.gb/3915190‑3915250                                                    |          tGGACATGGAAGTCGGCGCGGGACCCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:192387/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGACCCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:705976/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:489215/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:986088/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:966154/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:946710/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:914096/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:867899/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:831330/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:80421/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:617569/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:568163/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:507060/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:496666/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:492749/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:1055101/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:463373/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:434686/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:399805/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:380414/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:317720/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:221961/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:166372/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:139499/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:1379555/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:137188/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGcc  >  1:1212579/1‑61 (MQ=255) tGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGc   >  1:1084759/1‑60 (MQ=255)                                                    |          TGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCC  >  W3110S.gb/3915190‑3915250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑