Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	408,185	A→C	intergenic (+11/+147)	ykiA → / ← rdgC	hypothetical protein/DNA‑binding protein, non‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	408,185	0	A	C	100.0%	122.2 / NA	44	intergenic (+11/+147)	ykiA/rdgC	hypothetical protein/DNA‑binding protein, non‑specific
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (0/44);  total (0/44)

CACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCAT  >  W3110S.gb/408151‑408212                                   |                            cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:66520/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:1009369/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:339191/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:382284/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:397781/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:426269/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:455456/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:494664/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:599272/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:611353/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:62294/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:309225/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:715970/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:76710/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:799104/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:887171/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:898118/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:931064/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:931768/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:978205/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:988888/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:1367168/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:1012109/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:1027251/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:1068508/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:1097332/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:1163403/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:1251729/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:127591/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:298887/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:1400728/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:1437116/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:153022/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:172380/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:197131/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:204453/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:227937/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:228619/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:279769/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAACTTACAGCATAAAACGCat  <  1:1314627/62‑1 (MQ=255) cACCGGCTTGAGGAAAAACAATAACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:1184288/62‑1 (MQ=255)  aCCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGctt  <  1:679982/61‑3 (MQ=255)  aCCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:437667/61‑1 (MQ=255)  aCCGGCTTGAGGAAAAACAATGACACAACGCCCCTGGAGCAAGTTACAGCATAAAACGCat  <  1:1209123/61‑1 (MQ=255)                                   |                            CACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCAT  >  W3110S.gb/408151‑408212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑