Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 64,692 C→T V363V (GTG→GTA)  polB ← DNA polymerase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb64,6920CT100.0% 299.8 / NA 108V363V (GTG→GTA) polBDNA polymerase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (108/0);  total (108/0)

Only 100 of 168 total aligned reads displayed.

CGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTG  >  W3110S.gb/64679‑64751
             |                                                           
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGtc                                        >  1:613024/1‑34 (MQ=37)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCg                                   >  1:885277/1‑40 (MQ=37)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGa                              >  1:542154/1‑45 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCaaaaacg                     >  1:1053912/1‑52 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGa               >  1:1254779/1‑60 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGa               >  1:184232/1‑60 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGa               >  1:1352532/1‑60 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGa               >  1:1009161/1‑60 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:307569/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:515269/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:499449/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:463200/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:453488/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:435295/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:412283/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:367846/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:352684/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:948190/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:273684/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:262831/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:258311/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:23932/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:211696/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:205922/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:190469/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:969087/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:694853/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:860323/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:814146/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:79091/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:788637/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:738481/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:73161/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:720705/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:710691/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:898433/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:685979/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:628901/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:923808/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:605846/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:593296/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1006325/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:582483/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:560830/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:106289/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1125671/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1157477/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1189580/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1203706/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:169711/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1251087/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1269309/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1278193/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1299131/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1064627/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1318933/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1358849/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:165606/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:155798/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1034325/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1001004/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:148740/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1429071/1‑61 (MQ=255)
cgccgTGTCGATCTACCGGCAGGCCGTTCACCGTCGCCCGTTCGAGCAAAAACGGCATGAt              >  1:1465849/1‑61 (MQ=255)
            ccACCGGCAGGCCTTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1033619/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:990540/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1050603/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1115896/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:752948/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:755816/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:776813/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1084276/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:947897/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:931176/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:814056/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1206730/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:852344/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1038436/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:10571/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:869477/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1385046/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:196133/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:228196/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1452966/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1412311/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:318348/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:333666/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:34220/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:344184/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:139852/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1146706/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1334933/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1308452/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1301611/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:599909/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1212032/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:677775/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:690328/61‑1 (MQ=255)
            ccACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1162443/61‑1 (MQ=255)
             cACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTtgtg  <  1:1327655/60‑1 (MQ=255)
             |                                                           
CGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTG  >  W3110S.gb/64679‑64751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑