Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,982,958	T→A	A155A (GCA→GCT)	hdeD ←	acid‑resistance membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,982,958	0	T	A	100.0%	91.4 / NA	34	A155A (GCA→GCT)	hdeD	acid‑resistance membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (34/0);  total (34/0)

AATATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACAGACACCATCGGCGTTGCA  >  W3110S.gb/3982902‑3982961                                                         |    aaTATCAGTTCAATTCCCACCAGTGTGGACACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:585675/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:787725/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:585339/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:599260/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:63425/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:666129/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:669019/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:673199/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:688385/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:553073/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:855346/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:871416/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:902090/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:902114/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:923656/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:972072/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:987457/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:179501/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:1014940/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:102515/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:1047080/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:1055005/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:1247690/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:130840/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:138045/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:1445953/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:1000253/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:194729/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:237767/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:278315/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:352107/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:360238/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:407806/1‑60 (MQ=255) aaTATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACCGACACCATCGGCGTAGCa  >  1:545066/1‑60 (MQ=255)                                                         |    AATATCAGTTCAATTCCCACCAGTGTGGAAACCAGCGTTACAGACACCATCGGCGTTGCA  >  W3110S.gb/3982902‑3982961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑