Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	416,923	T→C	T186T (ACT→ACC)	phoB →	DNA‑binding response regulator in two‑component regulatory system with PhoR (or CreC)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	416,923	0	T	C	100.0%	94.8 / NA	35	T186T (ACT→ACC)	phoB	DNA‑binding response regulator in two‑component regulatory system with PhoR (or CreC)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/0);  total (35/0)

ACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACG  >  W3110S.gb/416867‑416927                                                         |     aCGCATCCTGAGCGCTTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:1196377/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:716418/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:479218/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:515832/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:526221/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:541350/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:565489/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:60729/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:635404/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:65212/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:410346/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:754298/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:774751/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:83268/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:870890/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:927659/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:940983/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:968233/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:165561/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:1141472/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:117497/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:1189355/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:1205302/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:1234061/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:1309003/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:1353763/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:155979/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:445115/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:229743/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:252670/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:264160/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:275257/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:367391/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:369620/1‑61 (MQ=255) aCGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACCAACg  >  1:1135808/1‑61 (MQ=255)                                                         |     ACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAACG  >  W3110S.gb/416867‑416927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑