Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,996,995	G→A	V587V (GTG→GTA)	prlC →	oligopeptidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,996,995	0	G	A	100.0%	81.2 / NA	31	V587V (GTG→GTA)	prlC	oligopeptidase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/31);  total (0/31)

AAACTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTGCCA  >  W3110S.gb/3996958‑3996998                                      |    aaaCTCTGGCAGATATCAAGAAACTGGTTGCCGTGGTAccg  <  1:54023/41‑2 (MQ=37) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:260230/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:985157/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:90796/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:902642/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:828826/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:781188/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:775484/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:771787/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:730665/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:591676/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:539148/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:46790/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:406297/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:261401/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:1000296/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:150402/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:1461212/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:1390777/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:1361206/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:1328078/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:1305788/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:1290409/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:1288935/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:1238579/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:1205204/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:111655/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:1111248/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:1105043/41‑2 (MQ=255) aaaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:1103940/41‑2 (MQ=255)  aaCTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTAccg  <  1:832775/40‑2 (MQ=38)                                      |    AAACTCTGGCAGAAATCAAGAAACTGGTTGCCGTGGTGCCA  >  W3110S.gb/3996958‑3996998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑