| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,000,037 | G→A | D90D (GAC→GAT) | uspA ← | universal stress global response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,000,037 | 0 | G | A | 75.3% | 198.8 / 65.0 | 146 | D90D (GAC→GAT) | uspA | universal stress global response regulator |
| Reads supporting (aligned to +/- strand): ref base G (0/36); new base A (0/110); total (0/146) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
Only 100 of 146 total aligned reads displayed.
CAAACCACCAAATCCATATCGTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCA > W3110S.gb/3999981‑4000061 | caAACCACGAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:568829/62‑1 (MQ=255)caAACCACCAGATCCATATTGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:559190/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGTCCCAGATcgcc < 1:134846/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:351634/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:528383/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:523098/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:505558/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:49375/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:41589/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:398461/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:387238/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:58621/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:271971/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:259456/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:230917/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:198170/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:194536/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:188251/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:183220/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1470359/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1451638/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:760661/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:988860/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:988638/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:954888/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:947208/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:924730/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:923303/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:89656/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:895985/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:833255/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:830670/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1005586/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:730215/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:726200/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:691489/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:652514/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:637947/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:632583/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:622160/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:608772/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:595937/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1159866/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:128875/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1270008/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1256197/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1237104/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1436952/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1189816/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1184403/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1177848/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1162720/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1198324/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:114733/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1137253/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1129517/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:112754/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1118435/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1074500/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1054957/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1003373/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1370325/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1353691/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1352353/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1328152/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1332481/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1370494/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATagcc < 1:1160411/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCTCGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:354177/62‑1 (MQ=255)caAACCACCAGATCCATATCGTATTTCCTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:658657/62‑1 (MQ=255) aaaCCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:1349064/61‑1 (MQ=255) aaaCCACCAGATCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:618534/61‑1 (MQ=255) aTCCATATCGTATTTCTTGATTGCATCGACCAGTACCTGGCCCAGATcgcc < 1:607310/51‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCTAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:811313/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGGTGCCGCTCAGGGTTTCa < 1:757783/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:1406732/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:880810/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:1411679/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:1068313/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:865688/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:1418493/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:834586/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:1016738/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:1424550/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:237124/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:704571/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:801480/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:1147689/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:1148020/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:1304183/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:66845/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:349845/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:356879/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:1230383/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:1313066/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:1274803/61‑1 (MQ=255) gTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:428971/61‑1 (MQ=255) tATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:220410/60‑1 (MQ=255) cTTGATTGCATCGACCTGTACCTGGCCCAGATcgcc < 1:509627/36‑1 (MQ=25) aCCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCa < 1:1256299/36‑1 (MQ=255) | CAAACCACCAAATCCATATCGTATTTCTTGATTGCATCGACCAGAACCTGGCCCAGGTCGCCGCTGCCGCTCAGGGTTTCA > W3110S.gb/3999981‑4000061 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |