Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 439,953 A→G L125L (TTA→CTA)  ispA ← geranyltranstransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb439,9530AG99.2% 352.0 / ‑5.1 123L125L (TTA→CTA) ispAgeranyltranstransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (122/0);  minor base T (1/0);  total (123/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 123 total aligned reads displayed.

GCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATC  >  W3110S.gb/439935‑439996
                  |                                           
gCATATCGGCATCGCTTAGAATCGTGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:687566/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTaaa                    >  1:846137/1‑44 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAAt   >  1:385302/1‑61 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAAt   >  1:1451008/1‑61 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAAt   >  1:523052/1‑61 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:510576/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:615701/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:613318/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:602673/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:594029/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:585221/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:575072/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:574982/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:57197/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:546294/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:545550/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:636337/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:510441/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:486652/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:480037/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:422798/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:421896/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:384636/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:375848/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:351915/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:343453/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:324901/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:824591/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:986200/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:985830/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:980218/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:950313/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:911964/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:906368/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:857992/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:856469/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:850930/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:848273/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:833199/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:631882/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:810940/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:791719/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:785263/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:772257/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:756611/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:74418/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:735632/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:72779/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:67531/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:647858/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:320880/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1162846/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1001785/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1303263/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1302142/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1300821/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1262079/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1252838/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1251403/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1241583/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1232466/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1224909/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1198788/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1308130/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1160653/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1156382/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1148518/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1143656/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:112052/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1113404/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1095689/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1066682/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1065030/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1044752/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1021142/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1472572/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:312124/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:307944/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:29541/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:272129/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:264702/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:262605/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:213052/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:202600/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:200791/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:189135/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:150180/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1309981/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1470141/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:146466/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1448354/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1438391/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1429048/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1411654/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1373263/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1364235/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1352717/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1344071/1‑62 (MQ=255)
gCATATCGGCATCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACAAGCGAGAATc  >  1:1237852/1‑62 (MQ=255)
gCATATCGGCACCGCTTAGAATCGAGAACGCCAGCGTTTGTAAAGCGTCACCAGCGAGAATc  >  1:1102123/1‑62 (MQ=255)
                  |                                           
GCATATCGGCATCGCTTAAAATCGAGAACGCCAGCGTTTGTAAAGCGTCGCCAGCGAGAATC  >  W3110S.gb/439935‑439996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑