Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,017,703	T→C	N1175D (AAC→GAC)	rhsB ←	rhsB element core protein RshB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,017,703	0	T	C	100.0%	40.8 / NA	18	N1175D (AAC→GAC)	rhsB	rhsB element core protein RshB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/0);  total (18/0)

CTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCTGCGCACC  >  W3110S.gb/4017668‑4017729                                    |                           ctgcAGCTGCTGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTgcg      >  1:464775/1‑58 (MQ=11) ctgcAGCTGATGCGGGTTGTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGcacc  >  1:1296494/1‑62 (MQ=11) ctgcAGCTGATGCGGGTTCTCTTCATTCCGCAGGTCGCCCCATTCATCATATTCTGCGcac   >  1:934598/1‑61 (MQ=255) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTgcg      >  1:539256/1‑58 (MQ=255) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGcacc  >  1:1161732/1‑62 (MQ=25) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGcacc  >  1:1289886/1‑62 (MQ=25) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGcacc  >  1:165140/1‑62 (MQ=25) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGcacc  >  1:244722/1‑62 (MQ=25) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGcacc  >  1:427911/1‑62 (MQ=25) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGcacc  >  1:1115685/1‑62 (MQ=25) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGcacc  >  1:493216/1‑62 (MQ=25) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGcacc  >  1:740065/1‑62 (MQ=25) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGcacc  >  1:775682/1‑62 (MQ=25) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGcacc  >  1:841265/1‑62 (MQ=25) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGcacc  >  1:85158/1‑62 (MQ=25) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGcac   >  1:1143443/1‑61 (MQ=25) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGcac   >  1:5878/1‑61 (MQ=25) ctgcAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGcac   >  1:1002281/1‑61 (MQ=25)                                    |                           CTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCTGCGCACC  >  W3110S.gb/4017668‑4017729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑