| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,032,296 | T→C | L557L (TTA→TTG) | zntA ← | zinc, cobalt and lead efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,032,296 | 0 | T | C | 95.0% | 304.5 / 1.6 | 119 | L557L (TTA→TTG) | zntA | zinc, cobalt and lead efflux system |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base C (113/0); total (113/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.88e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
GCCCAGCGCGTTCAGTTCACTGATGGCAGTTGCAGCATCGGCGCGCAGGGTATCCTGTAACGCAATGACACCAAGCACGTCATCGTTACGTACTACCAGCACT > W3110S.gb/4032239‑4032341 | tcccAGCGCGTTCAGTTCACTGATGGCAGTTGCAGCATCGGCGCGCAGGGTATCCTGTAAc < 1:557178/60‑1 (MQ=255)gCCCAGCGCGTTCAGTTCACTGATGGCAGTTGCAGCATCGGCGCGCAGGGTATCCTGTAAc < 1:285230/61‑1 (MQ=255)gCCCAGCGCGTTCAGTTCACTGATGGCAGTTGCAGCATCGGCGCGCAGGGTATCCTGTAAc < 1:569835/61‑1 (MQ=255)gCCCAGCGCGTTCAGTTCACTGATGGCAGTTGCAGCATCGGCGCGCAGGGTATCCTGTAAc < 1:871385/61‑1 (MQ=255)gCCCAGCGCGTTCAGTTCACTGATGGCAGTTGCAGCATCGGCGCGCAGGGTATCCTGTAAc < 1:797729/61‑1 (MQ=255) cAGCGCGTTCAGTTCACTGATGGCAGTTGCAGCATCGGCGCGCAGGGTATCCTGTAAc < 1:671894/58‑1 (MQ=255) cgcgcAGGGTTTCCTGCAACGCAATGACACCCAGCACGTCATCATTAAGAACTACCAGCACt > 1:682868/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGCCACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:142597/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGCCACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:474372/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGt > 1:611920/1‑39 (MQ=37) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATcat > 1:656604/1‑43 (MQ=38) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACg > 1:606283/1‑49 (MQ=38) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTAcc > 1:401954/1‑56 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:414677/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:485265/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:957674/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:984001/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:469424/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:464217/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:453449/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:44484/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:431991/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:426026/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:497697/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:410833/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:390098/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:38195/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:374761/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:372167/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:354116/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:985306/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:64886/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:82165/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:850229/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:783842/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:73405/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:700695/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:855894/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:858369/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:671034/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:867838/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:83564/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:872339/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:610414/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:283246/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:933221/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:933659/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:556159/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:547915/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:541589/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1178444/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1003344/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1290629/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:128542/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1264862/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1252424/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1242422/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1239575/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1223358/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1207363/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1197146/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1182552/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1182135/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:131058/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:117374/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1160176/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1157522/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1149424/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1119335/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1103167/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:108001/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1064498/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1043517/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1030342/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1007411/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:15528/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:26318/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:248943/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:209846/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:20546/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:203466/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:198937/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:195707/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:178168/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:176508/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:172033/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:166299/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1440862/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1427161/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:141429/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1402956/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:139123/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1388092/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1387929/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1361714/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1358229/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1313357/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGAACTAACAGCACt > 1:187682/1‑62 (MQ=255) cgcgcAGGGTATCCTGCAACGCAAAGACACCCAGCACGTCATCATTACGAACTACCAGCACt > 1:1311146/1‑62 (MQ=255) cgcgcAGGGGATCCTGCAACGCAATGACACCCAGCACGTCATCATTACGaactacaa > 1:730447/1‑55 (MQ=255) | GCCCAGCGCGTTCAGTTCACTGATGGCAGTTGCAGCATCGGCGCGCAGGGTATCCTGTAACGCAATGACACCAAGCACGTCATCGTTACGTACTACCAGCACT > W3110S.gb/4032239‑4032341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |