Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,041,763 A→G V335V (GTA→GTG)  livJ → leucine/isoleucine/valine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,041,7630AG100.0% 246.6 / NA 88V335V (GTA→GTG) livJleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/88);  total (0/88)

AAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGC  >  W3110S.gb/4041731‑4041793
                                |                              
aaaTACCTGAAAGCGAACTCGGTTGATACGGTGATGGG‑CCGCTGACCTGGGATGAGAAAGGc  <  1:522772/62‑1 (MQ=25)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGTGGCCGCTGACCTGGGATGAGAAAGGc  <  1:148609/60‑1 (MQ=25)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGCAAGGc  <  1:1327679/60‑1 (MQ=25)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:499594/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:631387/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:614946/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:597415/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:562131/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:552014/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:536399/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:515474/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:514290/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:646225/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:453156/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:446142/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:428524/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:40877/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:389253/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:354329/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:324041/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:316021/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:305905/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:828106/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:990135/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:932684/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:92614/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:916013/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:898341/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:877235/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:869164/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:846769/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:84433/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:834674/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1044260/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:824058/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:779840/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:772066/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:758044/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:737148/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:732272/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:730914/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:706510/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:663501/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:12014/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1244249/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1241934/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1238652/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1235150/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1227600/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1219180/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1217878/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1215552/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1208932/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1205473/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:30182/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1095989/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1072654/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1059949/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1057923/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1055157/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1053467/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1047744/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1045856/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1040631/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1250523/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:3030/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:293328/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:26309/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:254827/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:199804/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:190058/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:171149/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1466609/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1456650/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1434776/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1421467/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1421016/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1415985/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1396239/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1379568/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1303224/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1258076/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:1245051/60‑1 (MQ=37)
   tACCTGAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCGGACCTGGGATGAGAAAGGc  <  1:673103/60‑1 (MQ=25)
   tACCTGAAAGCGAACTCGGGTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:755649/60‑1 (MQ=255)
        gAAAGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:13401/55‑1 (MQ=37)
          aaGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:994371/53‑1 (MQ=255)
           aGCGAACTCGGTTGATACGGTGATGGGGCCGCTGACCTGGGATGAGAAAGGc  <  1:406030/52‑1 (MQ=255)
                                |                              
AAATACCTGAAAGCGAACTCCGTGGATACCGTAATGGGACCGCTGACCTGGGATGAGAAAGGC  >  W3110S.gb/4041731‑4041793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑