| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,069,946 | T→C | D283D (GAT→GAC) | glgX → | glycogen debranching enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,069,946 | 0 | T | C | 97.1% | 273.9 / ‑3.1 | 102 | D283D (GAT→GAC) | glgX | glycogen debranching enzyme |
| Reads supporting (aligned to +/- strand): ref base T (3/0); new base C (99/0); total (102/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
CCTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGATAACCGTAGCTATTATTGGATAAGAGAAGACGGCGATTATCACAACTGGACCG > W3110S.gb/4069910‑4069998 | ccTCGGCGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:309566/1‑60 (MQ=255)ccTCGCCGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:460950/1‑60 (MQ=255)ccTCGCCGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:741060/1‑60 (MQ=255)ccTCGACGGCCCGTTATTGTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:609864/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTGGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:173337/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCtatta > 1:684469/1‑51 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTg > 1:610456/1‑54 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTg > 1:1439922/1‑54 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGa > 1:1093281/1‑56 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATaa > 1:1156351/1‑59 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATa > 1:466504/1‑58 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:378465/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:476668/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:411172/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:412354/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:425953/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:435307/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:594766/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:376128/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:368021/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:358083/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:307341/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:289594/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:288867/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:286969/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:273441/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:272319/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:244322/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:243523/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:812865/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:948487/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:944745/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:932713/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:922328/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:909628/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:866494/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:864560/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:84452/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:836554/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:828015/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:572260/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:77886/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:754889/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:741022/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:684984/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:670230/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:664409/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:625837/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:233218/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:594707/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:578087/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1168524/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1299613/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1296647/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1289061/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1275947/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:126904/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1240357/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1231913/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1231042/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1222376/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1196175/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1185590/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1336671/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1158679/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1155588/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1103043/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1091486/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1079343/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1078384/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1066790/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1066527/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1034889/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1032611/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1028645/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1391788/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:191914/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:190892/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:18259/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1740/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:16236/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:161799/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:15938/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:149842/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:148687/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1425365/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:102403/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1374739/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1367564/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1364652/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1355452/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1351705/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1351018/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1349806/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:1341687/1‑60 (MQ=255)ccTCGACGGCCCGTTATTCTAGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:562354/1‑60 (MQ=255) cTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGACAACCGTAGCTATTATTGGATAag > 1:896200/1‑59 (MQ=255) tGGGATCGATAACCGTAGCTATTATTGGATAAGAGAAGACGGCGATTATCACAACTGGACCg > 1:1396958/1‑62 (MQ=255) tGGGATCGATAACCGTAGCTATTATTGGATAAGAGAAGACGGCGATTATCACAACTGGACCg > 1:1350763/1‑62 (MQ=255) tGGGATCGATAACCGTAGCTATTATTGGATAAGAGAAGACGGCGATTATCACAACTGGACCg > 1:570491/1‑62 (MQ=255) | CCTCGACGGCCCGTTATTCTCGCTGCGTGGGATCGATAACCGTAGCTATTATTGGATAAGAGAAGACGGCGATTATCACAACTGGACCG > W3110S.gb/4069910‑4069998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |