Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,083,677	A→G	S37G (AGC→GGC)	rtcA →	RNA 3'‑terminal phosphate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,083,677	0	A	G	100.0%	67.0 / NA	25	S37G (AGC→GGC)	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/0);  total (25/0)

GCGCTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCAGCATTCGTGCCGGGCG  >  W3110S.gb/4083632‑4083693                                              |                 gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:1001767/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:890975/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:83990/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:822129/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:80758/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:621407/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:48905/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:410035/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:315129/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:214912/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:176468/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:1471591/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:1412090/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:1346914/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:1150192/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggc   >  1:241087/1‑61 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggc   >  1:250943/1‑61 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggc   >  1:584227/1‑61 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggc   >  1:657837/1‑61 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggc   >  1:755602/1‑61 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggc   >  1:1248027/1‑61 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggc   >  1:1025569/1‑61 (MQ=255) gcgcTGAGCCTGTCGATGATAACCGGCAAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:221228/1‑62 (MQ=255) gcgcTGAGCCTGTCGATGATAACCAGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:272143/1‑62 (MQ=255) gcgcTGAGCATGTCGATGATAACCGGCCAGCCATTTACCATCACCGGCATTCGTGCcgggcg  >  1:906730/1‑62 (MQ=255)                                              |                 GCGCTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCAGCATTCGTGCCGGGCG  >  W3110S.gb/4083632‑4083693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑