| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,152,194 | T→C | L478L (CTA→CTG) | yhfK ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,152,194 | 0 | T | C | 80.8% | 218.8 / 36.2 | 125 | L478L (CTA→CTG) | yhfK | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (0/24); new base C (101/0); total (101/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.11e-26 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 125 total aligned reads displayed.
CCAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTGCAGGGTATACACTGCGGTAATAGTAA > W3110S.gb/4152138‑4152225 | ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:850902/62‑1 (MQ=255)ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:757181/62‑1 (MQ=255)ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:197593/62‑1 (MQ=255)ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:593712/62‑1 (MQ=255)ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:219916/62‑1 (MQ=255)ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:1191100/62‑1 (MQ=255)ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:1156563/62‑1 (MQ=255)ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:1065903/62‑1 (MQ=255)ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:519560/62‑1 (MQ=255)ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:1003321/62‑1 (MQ=255)ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:682225/62‑1 (MQ=255)ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:488592/62‑1 (MQ=255)ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:437918/62‑1 (MQ=255)ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:551068/62‑1 (MQ=255)ccAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGATCAACCACAATAGTTg < 1:791206/62‑1 (MQ=255) cAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:1329881/61‑1 (MQ=255) cAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:1350378/61‑1 (MQ=255) aataatGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:182297/60‑1 (MQ=255) cGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTg < 1:1406775/49‑1 (MQ=255) atcGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1068393/3‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATGGTaa > 1:519882/2‑47 (MQ=25) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:601070/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:535656/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:627368/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:62990/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:588014/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:539896/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:538484/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:724016/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:534650/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:51977/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:504390/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:480504/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:473363/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:766421/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:97246/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:968900/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:927051/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:909885/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:874633/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:858594/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:858321/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:829713/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:808793/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:768454/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:665075/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:765795/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:760114/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:752311/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:749752/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:741651/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:740427/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:337918/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:711662/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:706982/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:670021/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1132465/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1413712/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1392356/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1388462/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1386100/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:132889/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1326133/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1317911/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1316996/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1304545/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1277243/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:142888/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1111360/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1076211/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1072867/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1062453/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1053384/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1046580/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1044391/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1036585/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:102673/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1024041/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:225664/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:444765/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:395000/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:34443/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:33346/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:316096/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:300682/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:282073/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:265677/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:457659/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:20449/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:204049/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:185768/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:184665/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:166278/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:165486/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:162565/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:162246/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTaa > 1:1441654/2‑47 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGGTAATAGTa > 1:161102/2‑46 (MQ=37) accGTTCAGCCACAACAGTTGCAGGGTATACACTGCGCTAATAGTaa > 1:494297/2‑47 (MQ=25) accGTTCAGACACAACAGTTGCAGGGTATACACTGCGGTaa > 1:276769/2‑41 (MQ=25) | CCAATAATGGTATCGATAAGACGCGGAAGGATGTATTGCTCGCCGTTCAACCACAATAGTTGCAGGGTATACACTGCGGTAATAGTAA > W3110S.gb/4152138‑4152225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |