Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,171,162	T→G	S200A (TCA→GCA)	chiA →	periplasmic endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,171,162	0	T	G	100.0%	225.7 / NA	79	S200A (TCA→GCA)	chiA	periplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (79/0);  total (79/0)

CAGCCTTCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTCAGCCAGC  >  W3110S.gb/4171156‑4171217       |                                                        caGCCTGCACCGGATGTGAAACCGGCCCCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1144958/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:492914/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:693017/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:681167/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:638301/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:625885/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:60745/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:57415/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:547080/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:511924/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:501490/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:723934/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:484709/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:478190/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:470089/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:460702/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:443613/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:438480/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:434279/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:430721/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:878662/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:994861/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:974713/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:930972/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:923033/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:907546/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:900895/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:898054/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:889905/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:887331/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:718872/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:859846/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:840352/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:837818/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:837372/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:801011/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:786413/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:77/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:41958/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1184998/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1012179/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1271061/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1266410/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1263517/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1236633/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1217300/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1209614/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1209466/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1187370/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1271093/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1179441/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1176803/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1167088/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:116402/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:115317/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1125225/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1080197/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1076831/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1436003/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:333676/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:327988/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:320869/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:319868/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:301404/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:30127/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:217176/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:168296/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:154699/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1294466/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1434230/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1411265/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1407772/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1392734/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1368318/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1337917/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccagc  >  1:1298677/1‑62 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccag   >  1:834140/1‑61 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTcagccag   >  1:81813/1‑61 (MQ=255) caGCCTGCACCGGATGTGAAACCGGCACCCGACGTTAAACCGGCTCCTCATCTTcagccagc  >  1:175340/1‑62 (MQ=255)       |                                                        CAGCCTTCACCGGATGTGAAACCGGCACCGGACGTTAAACCGGCTCCTGATGTTCAGCCAGC  >  W3110S.gb/4171156‑4171217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑