Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,192,121	A→G	intergenic (+17/‑148)	rpsQ → / → rplN	30S ribosomal subunit protein S17/50S ribosomal subunit protein L14

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,192,121	0	A	G	100.0%	161.1 / NA	57	intergenic (+17/‑148)	rpsQ/rplN	30S ribosomal subunit protein S17/50S ribosomal subunit protein L14
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (57/0);  total (57/0)

ACACTCTCTCAATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATCC  >  W3110S.gb/4192111‑4192172           |                                                    acacTCTCTCGATACGAATAAACGGCTCAGAAATGAg                           >  1:774735/1‑37 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCg                        >  1:796566/1‑40 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGttt                     >  1:351253/1‑43 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTAtttttt              >  1:1406092/1‑50 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTAtttttt              >  1:1074836/1‑50 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTAtttt                >  1:732949/1‑48 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTAttt                 >  1:432096/1‑47 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTAtt                  >  1:910194/1‑46 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTAccc        >  1:680221/1‑56 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTAccc        >  1:1068523/1‑56 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCatata   >  1:184433/1‑60 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCata     >  1:1195076/1‑59 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCata     >  1:1380809/1‑59 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCa       >  1:815083/1‑57 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCa       >  1:1033534/1‑57 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:849774/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:432852/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:525923/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:383506/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:1020560/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:313298/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:643631/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:214633/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:1177634/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:1220312/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:17486/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:153943/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:1470117/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:961329/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:1455003/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:1233892/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATcc  >  1:1288937/1‑62 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:629024/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:583934/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:550326/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:434880/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:991541/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:388446/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:1028681/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:1092994/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:1295426/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:1374507/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:1463420/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:210636/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:288712/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:344508/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:380529/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:388090/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATc   >  1:389700/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACACatat    >  1:1337927/1‑60 (MQ=39) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTAACCATATc   >  1:905250/1‑61 (MQ=255) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTATAccc        >  1:967578/1‑56 (MQ=39) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTATAacc        >  1:1050494/1‑56 (MQ=38) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTATACCCatatac  >  1:206135/1‑60 (MQ=39) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTATACCCata     >  1:304643/1‑59 (MQ=39) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTATACCCa       >  1:662349/1‑57 (MQ=39) acacTCTCTCGATACGAATAAACGGCTCAGAAATGAGCCGTTCATTTTTTCTACCCATATc   >  1:761036/1‑61 (MQ=255)           |                                                    ACACTCTCTCAATACGAATAAACGGCTCAGAAATGAGCCGTTTATTTTTTCTACCCATATCC  >  W3110S.gb/4192111‑4192172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑