Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,242,269 G→T A348A (GCG→GCT)  yjbH → predicted porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,242,2690GT78.4% 292.5 / 83.7 163A348A (GCG→GCT) yjbHpredicted porin
Reads supporting (aligned to +/- strand):  ref base G (35/0);  new base T (127/0);  total (163/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 163 total aligned reads displayed.

TACCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAA  >  W3110S.gb/4242218‑4242307
                                                   |                                      
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTATTCGGATc                               >  1:612290/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGAt                                >  1:428060/2‑60 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGAt                                >  1:799544/2‑60 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:340303/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:503892/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:492093/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:476293/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:467091/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:452013/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:418734/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:416894/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:381808/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:366369/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:541733/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:325684/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:317228/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:311714/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:305527/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:296923/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1011246/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:242784/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:239718/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:232406/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:222104/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:770954/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:979139/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:976844/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:976658/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:971535/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:9457/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:912928/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:881000/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:87153/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:829533/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:829321/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:535696/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:768565/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:762555/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:753732/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:73199/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:731883/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:719084/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:697723/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:690318/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:586263/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:273579/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1258773/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1387661/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1375026/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1329208/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:110810/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1301051/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1293089/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:12875/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1264780/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:159768/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1228821/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1227190/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1218356/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1209154/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1203867/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1177708/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:113633/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1440429/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:152656/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1038207/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1464584/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1453189/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1052861/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:216243/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1064013/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1085998/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1436719/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1436596/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1418432/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1408439/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1089924/2‑61 (MQ=255)
caCCGGCGAGCAGGTGAAATATCGTGAATCGCGCGAAGGGATCATCCGTGCTAATCGGATc                               >  1:1120184/2‑61 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGtgatg                         >  1:553331/1‑39 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:1014000/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:1143588/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:1127403/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:1117131/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:111027/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:920206/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:1156481/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:73823/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:1184883/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:590416/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:1236311/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:50627/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:1322945/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:414565/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:401944/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:320100/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:1443490/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:14823/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:157363/1‑62 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCa   >  1:32336/1‑61 (MQ=255)
                            tCGCGCGAAGGGATCATCCGTGCGAATCCGATCGTGATGAACGATCTGCCGGATGGGATCaa  >  1:109788/1‑62 (MQ=255)
                                                   |                                      
TACCGGCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAA  >  W3110S.gb/4242218‑4242307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑