Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,298,228	T→C	I52I (ATT→ATC)	gltP →	glutamate/aspartate:proton symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,298,228	0	T	C	98.4%	164.7 / ‑5.7	61	I52I (ATT→ATC)	gltP	glutamate/aspartate:proton symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (60/0);  minor base G (1/0);  total (61/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATTAA  >  W3110S.gb/4298169‑4298230                                                            |   cgcgCCTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1227830/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATGaa  >  1:1075471/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:460590/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:61852/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:585672/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:552342/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:544778/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:511896/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:497329/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:489419/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:649529/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:457287/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:455782/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:44958/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:447654/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:432042/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:421820/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:6237/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:41843/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:660774/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:703949/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:716687/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:718648/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:723048/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:782202/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:868362/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:887005/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:912104/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:91823/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:924267/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:935642/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:965372/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1325164/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1041042/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1044171/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1067169/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:110414/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1107290/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1123720/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1131423/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1168163/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1206515/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1210368/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1226087/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1259647/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1313228/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1022288/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:133311/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1350399/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1382930/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1390704/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:1427454/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:144126/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:167541/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:227974/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:256735/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:33650/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:381232/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:387633/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:400630/1‑62 (MQ=255) cgcgACTGGCTGGTCGTCAATTTGATCTCTCCGGCGGGTGATATCTTCATCCATCTGATCaa  >  1:837550/1‑62 (MQ=255) cgcgACTGACTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATaaa  >  1:1470387/1‑62 (MQ=255)                                                            |   CGCGACTGGCTGGTCGTCAATTTGCTCTCTCCGGCGGGTGATATCTTCATCCATCTGATTAA  >  W3110S.gb/4298169‑4298230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑