Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,315,199 T→C L154L (CTA→CTG)  alsA ← fused D‑allose transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,315,1990TC99.2% 348.5 / ‑5.1 124L154L (CTA→CTG) alsAfused D‑allose transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (123/0);  minor base A (1/0);  total (124/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 124 total aligned reads displayed.

ATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAA  >  W3110S.gb/4315172‑4315225
                           |                          
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTg                    >  1:214931/2‑36 (MQ=37)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGc                   >  1:376414/2‑37 (MQ=37)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTgg             >  1:749027/2‑43 (MQ=38)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTgatagata   >  1:781895/2‑53 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTgatagata   >  1:506916/2‑53 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTgatagata   >  1:863136/2‑53 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTgatagata   >  1:819432/2‑53 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:515820/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:655995/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:647742/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:643533/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:615528/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:590917/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:575914/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:570499/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:556109/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:548215/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:520279/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:739701/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:509954/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:50815/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:503181/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:476540/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:475475/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:473416/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:472999/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1040420/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:849353/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:994850/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:971383/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:962333/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:960960/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:945931/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:927908/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:910866/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:896739/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:885601/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:879946/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:875288/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:680847/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:838999/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:836180/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:820119/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:819513/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:808080/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:805568/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:797594/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:780031/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:454225/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:71990/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1178495/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:132314/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:40514/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1310958/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1310407/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1258518/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1256081/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1255866/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1255721/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1220839/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1211467/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:11979/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1322625/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1150720/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1150078/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1125389/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:110499/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1094765/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1080439/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1073302/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1054173/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1053167/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1044997/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:195798/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:40425/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:374746/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:32542/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:313261/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:3034/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:292609/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:246652/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:223951/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:213321/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:197548/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1371920/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:184808/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:184405/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:170813/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1470962/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:144401/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1416433/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1401458/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1395617/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1376773/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1372843/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTTGTGGCTAATAGATaa  >  1:1145831/2‑54 (MQ=38)
gtCGAGCATCAGCGTTTTGGCAATTTCCAGCATCTGCTAGTGGCTgatagat    >  1:704282/2‑52 (MQ=38)
gtCGAGCATCAGCGTTTTGGCAATTTCAAGCATCTGCTTGTGGCTGATAGATaa  >  1:84592/2‑54 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAATTTACAGCATCTGCTTGTGGCTgatagat    >  1:877246/2‑52 (MQ=39)
gtCGAGCATCAGCGTTTTGGCAAATTCCAGCATCTGCTTGTGGCTGATAGATaa  >  1:1063137/2‑54 (MQ=39)
                           |                          
ATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAA  >  W3110S.gb/4315172‑4315225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑