Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,320,415	G→A	V116V (GTC→GTT)	phnN ←	ribose 1,5‑bisphosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,320,415	0	G	A	100.0%	238.0 / NA	85	V116V (GTC→GTT)	phnN	ribose 1,5‑bisphosphokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (85/0);  total (85/0)

CCACGGTTTTCCAGGCGCTGGCGGAGGATCTCCGGCGAAACCTGTAAACAGACGGGCAGCAG  >  W3110S.gb/4320365‑4320426                                                   |            ccATGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1204503/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAACCCTGTAAACAAACGGGcagcag  >  1:1197529/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGgcagca   >  1:1029768/1‑61 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGgcagca   >  1:929716/1‑61 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGgcagca   >  1:633634/1‑61 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGgcagca   >  1:525148/1‑61 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGgcagca   >  1:41093/1‑61 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGgcagca   >  1:356990/1‑61 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGgcagca   >  1:293958/1‑61 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGgcagca   >  1:1403901/1‑61 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1043351/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:649382/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1037517/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:602767/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:584684/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:583986/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:557684/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:538726/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:538336/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:532566/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:675780/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:486214/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:474431/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:453465/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:450936/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:437095/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:422521/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1053367/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:396835/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:795214/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:967330/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:958235/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:954332/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1024720/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:90733/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:855628/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:826920/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:811239/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:802075/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1189495/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:770231/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:745492/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:743563/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:742657/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:738379/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:736571/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:697165/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:681455/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1337864/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1448875/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:142140/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1074692/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1388333/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1375387/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1365015/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1360940/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1346924/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1343278/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1457990/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1316704/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1316270/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:128420/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1243408/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1212912/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1088486/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1145704/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:116916/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:238603/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:360497/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1073216/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:335655/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:317718/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:1074314/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:280833/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:243450/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:242273/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:215769/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:211831/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:209125/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:191192/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:177507/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:17296/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:172026/1‑62 (MQ=255) ccACGGTTTTCCAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:157403/1‑62 (MQ=255) ccACGGTTTTCAAGGCGCTGGCGCAGGATCTCTGGCGAAACCTGTAAACAAACGGGcagcag  >  1:394219/1‑62 (MQ=255)                                                   |            CCACGGTTTTCCAGGCGCTGGCGGAGGATCTCCGGCGAAACCTGTAAACAGACGGGCAGCAG  >  W3110S.gb/4320365‑4320426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑