Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,321,596	C→T	V101V (GTG→GTA)	phnM ←	carbon‑phosphorus lyase complex subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,321,596	0	C	T	100.0%	247.2 / NA	89	V101V (GTG→GTA)	phnM	carbon‑phosphorus lyase complex subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (89/0);  total (89/0)

GCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCAC  >  W3110S.gb/4321549‑4321598                                                |   gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:439845/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:589519/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:580378/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:577216/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:556127/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:550334/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:505021/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:493111/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:475408/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:468262/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:463496/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:610802/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:437308/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:433216/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:429586/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:425521/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:425441/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:398073/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:397336/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:395401/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:350977/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:34319/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:79570/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:922447/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:890764/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:877116/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:858655/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:838970/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:823117/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:812790/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:809273/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:80102/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:79744/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:336102/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:786746/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:774165/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:758690/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:710289/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:69664/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:694866/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:691626/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:689160/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:645040/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:637500/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1239809/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1367141/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1330666/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:131262/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1312562/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1287808/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1279653/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1273334/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1266612/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:124972/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1245819/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:124042/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:138596/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:120599/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1192259/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1145759/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1145445/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:112808/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1127032/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1111249/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1080734/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1076249/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1039247/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:195293/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:323377/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:311801/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:30709/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:283574/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:276624/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:260550/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:25762/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:248502/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:221148/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:216562/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1014384/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:188763/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:186738/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:181718/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1462557/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1442218/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1437158/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1414996/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1402112/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGTAc  >  1:1395330/1‑50 (MQ=255) gCGTTGATCATCTTCTCCAGATTCTCAAGCCGATCGCCGCCGTCGCGTAc  >  1:476174/1‑50 (MQ=38)                                                |   GCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCAC  >  W3110S.gb/4321549‑4321598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑