Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,347,217	G→A	E209E (GAG→GAA)	melA →	alpha‑galactosidase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,347,217	0	G	A	100.0%	154.6 / NA	57	E209E (GAG→GAA)	melA	alpha‑galactosidase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/57);  total (0/57)

CGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCAAAACCGCCGACG  >  W3110S.gb/4347179‑4347239                                       |                       cGTTGTGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:876412/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:635304/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:101037/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:339959/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:344299/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:376026/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:378610/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:397095/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:430531/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:488209/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:507063/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:539573/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:5584/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:578749/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:587896/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:303337/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:637306/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:67540/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:68194/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:685195/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:691056/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:792640/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:841378/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:851138/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:884386/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:910295/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:913076/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:961843/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1326719/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1010447/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1018516/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:102677/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1089535/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1093580/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1124214/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1129470/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1136371/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1152640/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1185642/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1241908/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1269715/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:296958/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:140281/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1406949/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1449018/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1461386/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:170421/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:234294/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:237111/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:249713/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:256245/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:25999/61‑1 (MQ=255) cGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:280179/61‑1 (MQ=255) cGTTGCGCAGATATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:377045/61‑1 (MQ=255)  gTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:192356/60‑1 (MQ=255)  gTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:840047/60‑1 (MQ=255)                     tatGGCGTTTTACCTGGAACTGGAGCGCAAAACCGCCGACg  <  1:1278173/41‑1 (MQ=255)                                       |                       CGTTGCGCAGGTATCAACCATATGGCGTTTTACCTGGAGCTGGAGCGCAAAACCGCCGACG  >  W3110S.gb/4347179‑4347239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑