Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,350,102	T→G	Q46P (CAG→CCG)	yjdF ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,350,102	0	T	G	98.2%	154.3 / ‑4.5	56	Q46P (CAG→CCG)	yjdF	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (55/0);  minor base C (1/0);  total (56/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

GCAGCGGCGTTAACGGATAACGTCTGGCGGTGGCAAGCAGTAGCTGCACGACAATAATCACC  >  W3110S.gb/4350058‑4350119                                             |                  gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:660619/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:148806/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:150367/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:29107/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:328288/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:360904/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:4293/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:448104/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:523503/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:556424/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:633207/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:645269/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:647861/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:278578/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:674039/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:701148/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:738434/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:765336/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:794564/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:870016/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:875139/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:885902/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:890708/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:909078/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:973112/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1272233/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1040568/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1073130/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1095267/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:111020/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1112328/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1119731/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1172042/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1175142/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1255949/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1264523/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1264538/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1265180/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1428265/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1300585/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1419307/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1417607/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:141631/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1413853/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1366314/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1365132/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:1343600/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAca  >  1:1370267/1‑61 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAc   >  1:719133/1‑61 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAc   >  1:485582/1‑61 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAc   >  1:1006757/1‑61 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAc   >  1:150449/1‑61 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAc   >  1:928088/1‑61 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGCCGCACGACAATAATCAcc  >  1:1272937/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTTAGCGGTGGCAAGCAGTAGAGGCACGACAATAATCAcc  >  1:701758/1‑62 (MQ=255) gcagcGGCGTTAACGGATAACGTTAAGCGGTGGCAAGCAGTAGCGGCACGACAATAATCAcc  >  1:312081/1‑62 (MQ=255)                                             |                  GCAGCGGCGTTAACGGATAACGTCTGGCGGTGGCAAGCAGTAGCTGCACGACAATAATCACC  >  W3110S.gb/4350058‑4350119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑