Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,354,619 T→C T32T (ACA→ACG)  dcuR ← DNA‑binding response regulator in two‑component regulatory system with DcuS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,354,6190TC100.0% 285.4 / NA 100T32T (ACA→ACG) dcuRDNA‑binding response regulator in two‑component regulatory system with DcuS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (100/0);  total (100/0)

CTCCAGCGTCGAGGCTGTTCCACAGCATTGAAAGCCTGGGATTTGTGCTACGTATCGGCGA  >  W3110S.gb/4354604‑4354664
               |                                             
ctcCAGCGTTGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:312533/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGccc                          >  1:1238085/1‑36 (MQ=38)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGccc                          >  1:455040/1‑36 (MQ=38)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCgg                        >  1:544539/1‑39 (MQ=37)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTa         >  1:1031920/1‑54 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATcgacga  >  1:110952/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCgg     >  1:322075/1‑58 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCg   >  1:116896/1‑60 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:476817/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:46249/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:476848/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:477063/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:481084/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:499295/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:532082/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:542794/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:546308/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:578880/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:466523/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:4628/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:611363/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:43481/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:412565/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:405593/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:399783/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:379287/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:354896/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:351818/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:761417/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1004308/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:9820/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:940741/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:905075/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:894809/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:878834/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:872556/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:868314/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:86497/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:789337/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:773014/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:595366/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:748557/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:747588/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:73754/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:713412/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:696966/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:683439/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:655518/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:636285/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:627603/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:998577/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:607880/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1214762/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:345452/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1358477/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1357770/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1355978/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1349859/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1296785/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1294152/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1291718/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:128660/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1281979/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1241332/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:136588/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1150329/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1140660/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1109888/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1092684/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1073516/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1046197/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1024717/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1024421/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1018010/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1016087/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1421369/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:328852/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:316282/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:301941/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:298874/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:284191/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:200105/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:19962/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:183632/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:170519/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:144114/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1434093/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1370245/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1417681/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1412033/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1387187/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1386916/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1378706/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1376960/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:137553/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1370634/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1370359/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGAGTTGTGCCACGTATCGGCGa  >  1:49514/1‑61 (MQ=255)
ctcCAGCGTCCAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:1324068/1‑61 (MQ=255)
ccccAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa  >  1:884229/3‑61 (MQ=255)
               |                                             
CTCCAGCGTCGAGGCTGTTCCACAGCATTGAAAGCCTGGGATTTGTGCTACGTATCGGCGA  >  W3110S.gb/4354604‑4354664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑