Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,354,640 T→C P25P (CCA→CCG)  dcuR ← DNA‑binding response regulator in two‑component regulatory system with DcuS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,354,6400TC100.0% 277.7 / NA 98P25P (CCA→CCG) dcuRDNA‑binding response regulator in two‑component regulatory system with DcuS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (98/0);  total (98/0)

Only 100 of 128 total aligned reads displayed.

CTCCAGCGTCGAGGCTGTTCCACAGCATTGAAAGCCTGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGATAATT  >  W3110S.gb/4354604‑4354700
                                    |                                                            
ctcCAGCGTTGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:312533/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCgg                                                            >  1:544539/1‑39 (MQ=37)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTa                                             >  1:1031920/1‑54 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATcgacga                                      >  1:110952/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCgg                                         >  1:322075/1‑58 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:354896/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:481084/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:476848/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:476817/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:466523/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:46249/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:43481/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:412565/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:405593/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:399783/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:379287/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:284191/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:351818/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:345452/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:316282/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:301941/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:298874/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1016087/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:73754/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:9820/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:940741/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:894809/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:878834/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:872556/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:86497/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:773014/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:761417/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:748557/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:499295/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:696966/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:683439/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:655518/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:636285/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:611363/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:607880/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:595366/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:546308/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:532082/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1294152/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:137553/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1046197/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1370634/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1370245/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:136588/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1358477/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1357770/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1349859/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:200105/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1291718/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:128660/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1241332/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1214762/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1092684/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1150329/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1140660/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1378706/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1004308/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:183632/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1018010/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:170519/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1024421/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:144114/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1434093/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1421369/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1376960/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1386916/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1387187/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1417681/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1412033/1‑61 (MQ=255)
ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGAGTTGTGCCACGTATCGGCGa                                      >  1:49514/1‑61 (MQ=255)
ctcCAGCGTCCAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:1324068/1‑61 (MQ=255)
ccccAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa                                      >  1:884229/3‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGcgtc               >  1:1052336/1‑48 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:786645/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:79373/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:1077399/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:1070073/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:995425/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:111338/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:73886/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:1132918/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:734834/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:1181291/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:626198/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:1290328/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:555185/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:1341202/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:453106/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:433812/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:32868/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:303145/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:173372/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:263974/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt  >  1:204898/1‑61 (MQ=255)
                                    tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaat   >  1:797412/1‑60 (MQ=255)
                                    |                                                            
CTCCAGCGTCGAGGCTGTTCCACAGCATTGAAAGCCTGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGATAATT  >  W3110S.gb/4354604‑4354700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑