| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,354,652 | T→C | V21V (GTA→GTG) | dcuR ← | DNA‑binding response regulator in two‑component regulatory system with DcuS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,354,652 | 0 | T | C | 76.4% | 184.5 / 49.5 | 127 | V21V (GTA→GTG) | dcuR | DNA‑binding response regulator in two‑component regulatory system with DcuS |
| Reads supporting (aligned to +/- strand): ref base T (30/0); new base C (97/0); total (127/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-03 | |||||||||||
Only 100 of 127 total aligned reads displayed.
CTCCAGCGTCGAGGCTGTTCCACAGCATTGAAAGCCTGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGATAATT > W3110S.gb/4354604‑4354700 | ctcCAGCGTTGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:312533/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTa > 1:1031920/1‑54 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATcgacga > 1:110952/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCgg > 1:322075/1‑58 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:399783/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:713412/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:73754/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:379287/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:354896/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:345452/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:328852/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:748557/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:761417/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:298874/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:773014/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:284191/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:789337/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:200105/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:19962/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:183632/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:144114/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:532082/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:636285/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:627603/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:611363/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:607880/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:595366/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:655518/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:546308/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:542794/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:412565/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:481084/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:477063/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:476848/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:466523/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:4628/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:46249/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:683439/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:86497/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:940741/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1296785/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1434093/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1291718/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1281979/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1241332/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1214762/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:894809/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1150329/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1140660/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1294152/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1109888/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1092684/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:9820/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1004308/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1046197/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1024421/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1018010/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1016087/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1349859/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1417681/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1412033/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1387187/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:872556/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1378706/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1376960/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:137553/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1370359/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1370245/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:136588/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1358477/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:1355978/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:878834/1‑61 (MQ=255)ctcCAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGAGTTGTGCCACGTATCGGCGa > 1:49514/1‑61 (MQ=255)ccccAGCGTCGAAGCCGTTCCACAGCATTGAAAGCCCGGGATTTGTGCCACGTATCGGCGa > 1:884229/3‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGcgtc > 1:1052336/1‑48 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCAt > 1:1413904/1‑53 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:73886/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:734834/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:79373/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:995425/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:173372/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:1070073/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:1077399/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:1132918/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:1181291/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:1290328/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:1341202/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:1379294/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:162998/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:555185/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:263974/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:297293/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:303145/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:304124/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:32868/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:379776/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:413193/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:433812/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaatt > 1:453106/1‑61 (MQ=255) tGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGAtaat > 1:797412/1‑60 (MQ=255) | CTCCAGCGTCGAGGCTGTTCCACAGCATTGAAAGCCTGGGATTTGTGCTACGTATCGGCGATTCAGCTCCGCGACCATTGCGTCGTCATCGATAATT > W3110S.gb/4354604‑4354700 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |