Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,416,028	A→T	P15P (CCA→CCT)	yjfK →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,416,028	0	A	T	100.0%	129.8 / NA	51	P15P (CCA→CCT)	yjfK	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (51/0);  total (51/0)

CAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGG  >  W3110S.gb/4416013‑4416051                |                        cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:785145/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:466940/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:505275/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:507463/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:542781/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:546804/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:557992/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:606307/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:689976/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:70394/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:706826/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:729041/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:730705/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:457650/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:795645/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:80605/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:811992/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:818807/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:819939/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:836992/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:837826/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:842635/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:848323/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:901507/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:980864/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:1348469/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:1057432/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:1123524/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:1124710/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:114784/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:1165481/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:1173462/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:120399/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:1207125/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:1223085/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:1316327/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:1340425/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:1048925/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:137456/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:1381682/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:1423969/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:159316/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:192475/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:285890/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:356007/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:363676/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:364551/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:385514/1‑39 (MQ=25) cAAGGATAATAAGCCTGCTATCGCTCGCGGTCCGTTggg  >  1:436035/1‑39 (MQ=25) cAAGGATAATAAGACTGCTATCGCTCGCGGTCCGTTggg  >  1:277598/1‑39 (MQ=25) cAAGGAGAATAAGCCTGCTATCGCTCGCGGTCCGTTg    >  1:803413/1‑37 (MQ=21)                |                        CAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGG  >  W3110S.gb/4416013‑4416051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑