Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,465,975 C→T V456V (GTG→GTA)  nrdD ← anaerobic ribonucleoside‑triphosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,465,9750CT100.0% 317.7 / NA 113V456V (GTG→GTA) nrdDanaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/113);  total (0/113)

Only 100 of 113 total aligned reads displayed.

TCATCGTCAGCATTGAGACGCACGCCACAAGCACCTTCCATATAGAGGATCGGGGCCACGCG  >  W3110S.gb/4465919‑4465980
                                                        |     
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:506922/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:626405/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:606968/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:587638/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:586291/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:577343/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:547050/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:542927/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:541645/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:537770/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:532158/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:510985/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:645462/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:505737/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:479320/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:459636/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:456763/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:455324/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:438721/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:43208/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:368319/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:364233/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1004642/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:355664/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:787310/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:994327/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:98802/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:984282/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:958458/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:953727/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:91363/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:897192/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:878825/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:859178/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:845452/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:814878/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:356469/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:783715/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:734704/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:726135/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:721978/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:69324/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:691741/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:689734/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:682337/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:681047/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:676554/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:665857/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1154405/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:351081/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1252162/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1230761/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1227291/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1218025/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1204612/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1195580/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1179337/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1178977/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1167684/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1161247/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1157785/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1273442/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1148862/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1143951/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1142583/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1124477/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1120785/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1099523/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1075254/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:107354/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:104879/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1037744/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1022344/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1006506/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1415932/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:298564/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:292726/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:305006/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:28275/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:238710/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:224343/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:222582/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:220623/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:174396/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1296202/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1419057/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1406376/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1388919/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1382104/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1380698/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1380447/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1325376/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1321255/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:13118/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:1298128/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:3381/59‑1 (MQ=255)
tcgtcGTCAGCATTCAGACGCACCCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:493372/59‑1 (MQ=255)
 cgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:366145/59‑1 (MQ=255)
 cgtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:645326/59‑1 (MQ=255)
  gtcGTCAGCATTCAGACGCACGCCACAAGCGCCTTCCATATAGAGGATCGGGGCTAcgcg  <  1:371765/59‑1 (MQ=255)
                                                        |     
TCATCGTCAGCATTGAGACGCACGCCACAAGCACCTTCCATATAGAGGATCGGGGCCACGCG  >  W3110S.gb/4465919‑4465980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑