Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,489,902	T→C	Q244Q (CAA→CAG)	pepA ←	aminopeptidase A, a cyteinylglycinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,489,902	0	T	C	100.0%	203.3 / NA	72	Q244Q (CAA→CAG)	pepA	aminopeptidase A, a cyteinylglycinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (72/0);  total (72/0)

CATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCT  >  W3110S.gb/4489887‑4489947                |                                              cATCAGCGATTCGTTCTGCGAACCCTGCCCGACCGCCAGAt                      >  1:140446/1‑41 (MQ=38) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAg                        >  1:1099716/1‑39 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGaa                 >  1:1247517/1‑46 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCTTCCCCAGctct  >  1:317335/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:660604/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:742497/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:660018/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:645910/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:609892/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:558517/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:534578/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:516078/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:512845/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:481263/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:43594/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:41956/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:410251/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:407983/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:37264/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:356432/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:827757/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:996446/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:996409/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:981391/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:957980/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:953285/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:925995/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:893301/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:845515/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:733748/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:820473/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:813756/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:805777/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:797967/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:77855/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:766903/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:75721/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:277203/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1117766/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1267864/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1249106/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1207860/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1168830/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1150630/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1136318/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:112898/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1127813/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1297499/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1099590/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:106033/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1044459/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1038029/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1028576/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1023878/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1007376/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1467642/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:100440/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:268533/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:249232/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:196209/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:19097/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:188302/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1468414/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:318983/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1411422/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1371761/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1361156/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1343666/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1324409/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1302913/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctct  >  1:1300673/1‑61 (MQ=255) cATCAGCGATTCGTTCTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGctc   >  1:148281/1‑60 (MQ=255)                |                                              CATCAGCGATTCGTTTTGCGAACCCTGACCGACCGCCAGATAGGAATGCATCCCCAGCTCT  >  W3110S.gb/4489887‑4489947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑