Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,572,746	G→A	I152I (ATC→ATT)	yjiO ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,572,746	0	G	A	100.0%	81.2 / NA	31	I152I (ATC→ATT)	yjiO	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/31);  total (0/31)

AGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCG  >  W3110S.gb/4572715‑4572753                                |        aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:404890/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:994641/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:871262/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:826652/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:825660/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:809680/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:649713/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:592947/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:587947/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:578249/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:575611/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:544111/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:49109/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:477180/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:1011013/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:404599/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:338370/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:272111/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:17401/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:1437986/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:1433503/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:1348141/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:1347095/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:1309841/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:1225939/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:1194109/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:1164798/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:1017119/39‑1 (MQ=255) aGTGCATCAGAGCTGCGCCGGAAAGCGGGCCAACAATCg  <  1:1327593/39‑1 (MQ=37) aGTGCAGCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:74935/39‑1 (MQ=37)  gTGCATCAGAGCTGCGCCGGAAAGCGGGCCAATAATCg  <  1:789839/38‑1 (MQ=255)                                |        AGTGCATCAGAGCTGCGCCGGAAAGCGGGCCGATAATCG  >  W3110S.gb/4572715‑4572753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑