Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,580,803	C→T	pseudogene (1987/2721 nt)	yjiV →	ECK4334:JW5954:b4486; conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,580,803	0	C	T	100.0%	78.8 / NA	30	pseudogene (1987/2721 nt)	yjiV	ECK4334:JW5954:b4486; conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/0);  total (30/0)

ATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCAC  >  W3110S.gb/4580753‑4580814                                                   |            aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCa   >  1:1098677/1‑61 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:273886/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:914072/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:911871/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:860066/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:712607/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:700600/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:69857/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:691202/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:669661/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:66732/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:661913/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:64943/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:45673/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:424490/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:33933/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:1039824/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:207201/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:190408/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:141221/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:1381024/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:1362014/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:1202817/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:1179938/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:1177855/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:1075352/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:1070357/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:1070201/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCAc  >  1:1043638/1‑62 (MQ=255) aTCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATCCCGGGGTCGGAGTGGCAc  >  1:1456735/1‑62 (MQ=255)                                                   |            ATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCAC  >  W3110S.gb/4580753‑4580814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑