Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	489,137	A→G	intergenic (+15/+197)	kefA → / ← ybaM	fused mechanosensitive channel proteins/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	489,137	0	A	G	100.0%	241.6 / NA	85	intergenic (+15/+197)	kefA/ybaM	fused mechanosensitive channel proteins/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (85/0);  total (85/0)

AGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTG  >  W3110S.gb/489116‑489177                      |                                         agtgTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:6395/4‑62 (MQ=37) agtgTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:237998/4‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTa                 >  1:1157572/1‑47 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACaaa            >  1:5018/1‑52 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAg           >  1:60467/1‑53 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGt          >  1:676503/1‑54 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:250174/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:361644/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:311566/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:305150/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:292852/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:87217/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:256059/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:473459/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:242259/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:887871/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:171083/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:385410/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:390171/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:426578/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:877059/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:51877/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:539869/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:596694/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:612829/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:620307/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:767282/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:756661/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:683660/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1452881/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1001678/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1044517/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:955655/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1104713/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:937657/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1134199/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1166033/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:936025/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1210537/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1228795/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1244560/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1252086/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1271203/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1276194/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1280767/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1332069/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1299623/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1357582/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1450535/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1361568/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:137003/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:14205/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:1407224/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:929450/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:916650/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgttg  >  1:137989/1‑62 (MQ=38) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:90724/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:749156/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:983741/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:859290/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:773586/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:798418/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:84882/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:818161/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:848731/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:279376/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:1035786/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:1094507/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:1107885/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:1206060/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:1377705/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:1459246/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:217130/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:735038/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:288489/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:299871/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:452235/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:478783/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:58993/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:633997/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:670689/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:67498/1‑61 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgt    >  1:531778/1‑60 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTTGCCCCGAGATTACTAACAAAGTGCGCgt    >  1:1375187/1‑60 (MQ=255) aGGGTAATAACCAAAGGGGGCGACATTTAGTAGCCCCGAGATTACTAACAAAGTGCGCgtt   >  1:215305/1‑61 (MQ=255)                      |                                         AGGGTAAAAACGAAAGGGGGCAACATTTAGTTGCCCCGAGATTGCTAACAAAGTGCGCGTTG  >  W3110S.gb/489116‑489177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑