Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	507,342	T→G	intergenic (‑38/‑100)	ybaP ← / → ybaQ	conserved hypothetical protein/predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	507,342	0	T	G	100.0%	139.4 / NA	50	intergenic (‑38/‑100)	ybaP/ybaQ	conserved hypothetical protein/predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (50/0);  total (50/0)

CAGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGTGAGGGATGGCGCAAA  >  W3110S.gb/507296‑507357                                               |                caGATCCATACGACCTCCCTTTGTGAAATATGATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:431883/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:384585/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1391906/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1403488/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1413927/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1449379/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:164153/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:190565/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:232978/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:238512/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:269922/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:287342/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:293356/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1026469/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:399631/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:534125/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:614196/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:615353/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:647545/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:648821/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:700478/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:748241/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:826964/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:902739/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:115475/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1008785/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1033739/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1035140/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1044287/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1048726/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:105399/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1077123/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1117971/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1127636/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1142154/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1385688/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1170740/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1179016/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1179239/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1187958/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1194106/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1241766/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1248055/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1268734/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:1345667/1‑62 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaa   >  1:1244795/1‑61 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaa   >  1:1360643/1‑61 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCaa   >  1:1391947/1‑61 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGGGAGGGATGGCGCa    >  1:721401/1‑60 (MQ=255) caGATCCATACGACCTCCCTTTGTGAAATATAATGCTAGCGCGCGGGGAGGGATGGCGCaaa  >  1:589608/1‑62 (MQ=255)                                               |                CAGATCCATACGACCTCCCTTTGTGAAATATCATGCTAGCGCGCGGTGAGGGATGGCGCAAA  >  W3110S.gb/507296‑507357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑