Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 534,474 C→T G445G (GGC→GGT)  gcl → glyoxylate carboligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb534,4740CT77.3% 207.1 / 56.4 141G445G (GGC→GGT) gclglyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base C (0/32);  new base T (109/0);  total (109/32)
Fisher's exact test for biased strand distribution p-value = 2.00e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

TGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGT  >  W3110S.gb/534433‑534523
                                         |                                                 
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:1308555/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:639734/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:694146/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:205872/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:218908/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:229260/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:782443/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:1383180/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:136827/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:586836/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:297698/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:835202/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:502767/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:351727/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:889986/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:891770/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:979174/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:360992/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:468057/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:95781/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:1155490/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:38911/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:937751/60‑1 (MQ=255)
tgtgCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTc                                 <  1:439877/60‑1 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTg                          >  1:1397846/1‑37 (MQ=25)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGcc               >  1:788481/1‑47 (MQ=37)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAg   >  1:485344/1‑60 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:354841/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:603174/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:602343/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:478062/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:569972/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:540457/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:517801/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:46356/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:517221/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:824459/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:98353/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:960839/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:938343/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:93617/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:925316/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:920044/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:862656/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:846549/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:842/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:828887/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:638369/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:810134/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:796045/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:792670/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:790091/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:774390/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:752605/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:745886/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:710140/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:698588/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1219030/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1353579/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1347722/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1344772/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1330072/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:131139/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1278281/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:126999/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1266627/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:123945/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1230742/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1405840/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:121467/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1191366/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1144687/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1122638/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1087648/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1080670/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1071768/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1069370/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1049816/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1035753/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:201791/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:440343/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:407783/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1013709/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:339410/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:336411/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:327799/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:277234/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:244501/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:237037/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:227933/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:458977/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:179046/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:177153/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:160901/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:153632/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1460009/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1456871/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1451664/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:141953/1‑61 (MQ=255)
                              gCGATTTCCGGTGACTTCGACTTCCAGTTCCTGATTGAAGAATTAGCCGTTGGCGCACAGt  >  1:1409366/1‑61 (MQ=255)
                                         |                                                 
TGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGT  >  W3110S.gb/534433‑534523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑