| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 80,688 | A→G | A59A (GCT→GCC) | leuC ← | 3‑isopropylmalate isomerase subunit, dehydratase component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 80,688 | 0 | A | G | 95.4% | 278.7 / ‑0.2 | 108 | A59A (GCT→GCC) | leuC | 3‑isopropylmalate isomerase subunit, dehydratase component |
| Reads supporting (aligned to +/- strand): ref base A (0/5); new base G (0/103); total (0/108) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
TCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCG > W3110S.gb/80660‑80730 | tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:384833/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:523754/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:514978/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:511428/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:491978/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:45189/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:450431/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:439230/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:433249/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:400575/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:38760/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:38509/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:532584/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:382229/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:36764/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:366677/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:339082/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:329760/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:315369/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1004284/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:267325/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:258734/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:254961/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:826005/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:988870/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:964075/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:959222/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:945660/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:931881/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:927281/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:896366/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:896247/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:887407/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:886062/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:874502/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:313251/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:818781/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:742249/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:719612/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:617641/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:595737/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:562366/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:556126/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:555910/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:542153/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:535102/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1146009/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1317121/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1264936/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1250349/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1248754/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1242676/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1241439/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1233411/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1203434/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1179734/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1170185/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1155403/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:249633/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1115957/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1114786/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:110254/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1099098/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1082529/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1078728/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1068984/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1053414/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:104952/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1013165/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1426483/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:160281/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:150285/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1469331/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:161130/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:192641/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1462029/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:208117/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1431281/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:209096/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1410897/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:222865/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1379213/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:236600/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:253801/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1328707/61‑1 (MQ=255)tCTGGGTAGAGACGTTGTGAGCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1015924/61‑1 (MQ=255) cTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1325491/60‑1 (MQ=255) cTGGGTAGAGACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:174854/60‑1 (MQ=255) agagACGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1176482/54‑1 (MQ=255) gagaCGTTGTGATCCATGGTGGCGAAGGTTTTCCCCGGCTGACGTACCGGGCg < 1:395333/53‑1 (MQ=39) gaCGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGgcg < 1:703265/61‑1 (MQ=255) gaCGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGgcg < 1:182763/61‑1 (MQ=255) gaCGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGgcg < 1:640932/61‑1 (MQ=255) gaCGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGgcg < 1:32650/61‑1 (MQ=255) gaCGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGgcg < 1:1392839/61‑1 (MQ=255) aCGTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:45576/50‑1 (MQ=255) gTTGTGATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:1268028/48‑1 (MQ=255) tgtgATCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:889872/46‑1 (MQ=255) aTCCATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:531500/42‑1 (MQ=255) cATGGTGGCGAAGGTTTTGCCCGGCTGACGTACCGGGCg < 1:434695/39‑1 (MQ=255) | TCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCG > W3110S.gb/80660‑80730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |