Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	549,452	G→T	L201L (CTG→CTT)	ylbF →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	549,452	0	G	T	100.0%	246.5 / NA	88	L201L (CTG→CTT)	ylbF	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (0/88);  total (0/88)

GATGCGCGCTCCGGTCGTCCGTTTTTTGCCTGTTCCGACAATCTGCAACTCGTTACCACAG  >  W3110S.gb/549408‑549468                                             |                 gATGCGCGCTCCGGTCGCGCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:69222/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:537100/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:906213/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:906447/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:691036/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:623566/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:614237/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:604808/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:555629/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:544372/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:733125/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:5334/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:527543/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:518244/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:481999/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:459755/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:449279/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:438546/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:432421/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:431178/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:805767/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:886744/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:885371/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:875513/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:8579/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:843253/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:840947/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:834359/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:822447/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:811686/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:429178/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:802935/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:77753/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:772727/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:767584/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:762744/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:750693/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:745567/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:736328/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1326266/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:422048/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:141958/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1417851/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:998751/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1385762/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1375161/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1373663/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1358707/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1344001/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1434068/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:13226/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1309736/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1253265/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1212101/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1201390/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1191909/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1176444/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:107788/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1020134/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1465141/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:947551/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:3571/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:355266/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:323524/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:315096/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:303855/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:948250/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:265841/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:260868/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:259142/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:233649/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:211018/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:169091/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:147496/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1466815/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:982918/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCGTTTTTTGCCTGATCCGACAATCTTCAACTCGTTACcactg  <  1:1342283/61‑3 (MQ=255) gATGCGCGCTCCGGTCGCCCG‑TTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:252752/60‑3 (MQ=255)  aTGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:900702/60‑3 (MQ=255)  aTGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1045242/60‑3 (MQ=255)  aTGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:732942/60‑3 (MQ=255)  aTGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:707581/60‑3 (MQ=255)  aTGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:370863/60‑3 (MQ=255)  aTGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:284755/60‑3 (MQ=255)  aTGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:146097/60‑3 (MQ=255)   tGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCGGACAATCTTCAACTCGTTACcactg  <  1:999032/59‑3 (MQ=255)   tGCGCGCTCCGGTCGCCCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:885979/59‑3 (MQ=255)              gtcgccCGTTTTTTGCCTGTTCCGACAATCTTCAACTCGTTACcactg  <  1:1386523/48‑3 (MQ=38)                                             |                 GATGCGCGCTCCGGTCGTCCGTTTTTTGCCTGTTCCGACAATCTGCAACTCGTTACCACAG  >  W3110S.gb/549408‑549468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑