| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,148,033 | 2 bp→CA | coding (2631‑2632/3318 nt) | yegE → | predicted diguanylate cyclase, GGDEF domain signalling protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,148,033 | 0 | T | C | 85.9% | 241.7 / 24.3 | 128 | G877G (GGT→GGC) | yegE | predicted diguanylate cyclase, GGDEF domain signalling protein |
| Reads supporting (aligned to +/- strand): ref base T (0/18); new base C (110/0); total (110/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.64e-22 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01 | |||||||||||
| * | W3110S.gb | 2,148,034 | 0 | G | A | 85.9% | 236.3 / 25.0 | 128 | V878I (GTC→ATC) | yegE | predicted diguanylate cyclase, GGDEF domain signalling protein |
| Reads supporting (aligned to +/- strand): ref base G (0/18); new base A (110/0); total (110/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.64e-22 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
AATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAA > W3110S.gb/2148007‑2148086 || aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:356867/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:577035/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:575646/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:57381/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:553955/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:539439/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:537954/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:535209/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:500561/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:448685/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:395105/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:580346/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:334616/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:33060/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:298672/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:286521/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:267939/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:264080/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:249189/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:22389/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:201324/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:172710/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:813030/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:964131/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:954498/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:9474/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:939707/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:909646/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:898263/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:894858/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:890597/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:840531/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:831207/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1009667/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:797226/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:760451/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:759376/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:740208/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:722232/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:692154/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:650559/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:632416/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:624983/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:601561/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1173514/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1336394/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1335099/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1313775/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1298676/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1297996/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1265799/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1257500/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1254889/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1230372/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1195932/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1463381/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1167314/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1159872/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1155670/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1094176/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1081671/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1069352/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1068918/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1039156/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:102165/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1004340/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1456802/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1412063/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1399643/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1414/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1450685/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1398044/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1395022/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1392538/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1455737/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1374033/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1373977/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:172703/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:136868/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1355361/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1347945/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggct > 1:1338609/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggc > 1:772921/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACggc > 1:1093483/1‑42 (MQ=25)aaTCAGTTGATGATGATCGCCCACGGCATCGCTTCGCCACg > 1:440082/1‑41 (MQ=25)aaTCAGTTGATGATGATCGCCCACCGCATCGCTTCGCCACggc > 1:1262810/1‑42 (MQ=21) ccACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTaa < 1:965243/60‑1 (MQ=255) ccACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTaa < 1:928864/60‑1 (MQ=255) ccACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTaa < 1:1021291/60‑1 (MQ=255) ccACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTaa < 1:1074493/60‑1 (MQ=255) ccACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTaa < 1:549502/60‑1 (MQ=255) ccACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTaa < 1:868439/60‑1 (MQ=255) ccACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTaa < 1:821604/60‑1 (MQ=255) ccACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTaa < 1:305178/60‑1 (MQ=255) ccACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTaa < 1:746748/60‑1 (MQ=255) ccACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTaa < 1:376221/60‑1 (MQ=255) ccACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTaa < 1:1203109/60‑1 (MQ=255) ccACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTaa < 1:620169/60‑1 (MQ=255) ccACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTaa < 1:471057/60‑1 (MQ=255) ccACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTaa < 1:561672/60‑1 (MQ=255) || AATCAGTTGATGATGCTCGCCCACGGTGTCGCTTCGCCACGGATCCCGGAAGCGCGTAATTTGTGGCTGATTTCACTTAA > W3110S.gb/2148007‑2148086 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |