| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,895,065 | 2 bp→GA | coding (548‑549/1278 nt) | yiaN ← | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,895,065 | 0 | T | G | 100.0% | 204.3 / NA | 77 | A183V (GCA→GTC) | yiaN | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (77/0); total (77/0) | |||||||||||
| * | W3110S.gb | 3,895,066 | 0 | G | A | 98.7% | 195.3 / NA | 77 | A183V (GCA→GTC) | yiaN | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (76/0); minor base C (1/0); total (77/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGGTAAGCATCAGCGTTGCGCCCATCATCATGCCAGGGGCAATGCCCGCCATAAACAGCT > W3110S.gb/3895047‑3895108 || ccaGGTAACCATCAGCGTGCCGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgt > 1:1420540/1‑60 (MQ=255)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTgg > 1:1003488/1‑39 (MQ=12)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:479803/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:633837/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:576780/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:543103/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:526428/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:511273/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:487539/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:483814/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:665567/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:462458/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:448688/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:410383/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:396005/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:356671/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:315947/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:269147/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:877611/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:98081/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:972301/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:971678/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:933248/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:930104/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:919728/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:916903/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:893335/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:285948/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:875254/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:837754/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:814985/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:789138/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:769404/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:769374/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:763250/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:736234/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1102480/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1232779/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:262602/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1192956/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1186509/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1177354/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:117352/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:113094/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:112954/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1122097/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1207619/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1088417/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1063623/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1054632/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1054313/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1049869/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1047822/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1036303/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:102904/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:143974/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:260185/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:184868/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:184730/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:180244/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:163507/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1465006/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1460964/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1427416/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1424920/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1407228/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1393075/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1390845/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:137471/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgtt > 1:1330829/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgt > 1:782822/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgt > 1:407513/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgt > 1:535524/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgt > 1:1047935/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgt > 1:945018/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGCCCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgt > 1:761833/1‑60 (MQ=25)ccaGGTAACCATCAGCGTGACGACCATCATGATGCCTGGCGCAATGCCCGCCATAAACAgt > 1:584860/1‑60 (MQ=255) || CCAGGTAAGCATCAGCGTTGCGCCCATCATCATGCCAGGGGCAATGCCCGCCATAAACAGCT > W3110S.gb/3895047‑3895108 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |