Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,619,282	2 bp→TA	intergenic (+340/+79)	yjjV → / ← yjjW	predicted DNase/predicted pyruvate formate lyase activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,619,282	0	C	T	100.0%	53.9 / NA	35	intergenic (+340/+80)	yjjV/yjjW	predicted DNase/predicted pyruvate formate lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/35);  total (0/35)
*	W3110S.gb	4,619,283	0	G	A	100.0%	53.9 / NA	35	intergenic (+341/+79)	yjjV/yjjW	predicted DNase/predicted pyruvate formate lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/35);  total (0/35)

GCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTA  >  W3110S.gb/4619264‑4619325                   ||                                           gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGGCGC‑TGACGCGTCTTATCAGGCCTa  <  1:260703/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:361157/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:951882/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:864908/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:822128/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:736292/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:727857/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:72439/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:695668/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:65949/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:612497/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:591230/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:414691/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:1027163/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:223111/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:1375147/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:1054105/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:1081937/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:1109311/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:1115871/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:1118325/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:1247639/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:1393142/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:1438040/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:1444052/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:1461220/61‑1 (MQ=11) gccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:156019/61‑1 (MQ=11)  ccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:612440/60‑1 (MQ=11)  ccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:637989/60‑1 (MQ=11)  ccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:186640/60‑1 (MQ=11)  ccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:11452/60‑1 (MQ=11)  ccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:578777/60‑1 (MQ=11)  ccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:494746/60‑1 (MQ=11)  ccgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:82092/60‑1 (MQ=11)   cgcATCCGGCAGTCGGTACACAATGCCTGATGCGACGC‑TGACGCGTCTTATCAGGCCTa  <  1:930874/59‑1 (MQ=11)                   ||                                           GCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTA  >  W3110S.gb/4619264‑4619325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑