| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,681,551 | Δ1 bp | coding (1653/1851 nt) | ilvD ← | dihydroxyacid dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,681,549 | 0 | G | . | 75.0% | 47.1 / 28.0 | 28 | coding (1655/1851 nt) | ilvD | dihydroxyacid dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (1/6); new base . (0/21); total (1/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
CGCTTCACGACGCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGC > W3110S.gb/3681491‑3681588 | cgcTTCACGACGCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGa > 1:1366918/1‑62 (MQ=255) ttACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAGGTCACCATCTTCAATc < 1:811433/62‑1 (MQ=255) ttACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAGGTCACCATCTTCAATc < 1:683972/62‑1 (MQ=255) ttACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAGGTCACCATCTTCAATc < 1:1907522/62‑1 (MQ=255) ttACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAGGTCACCATCTTCAATc < 1:2846189/62‑1 (MQ=255) ttACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAGGTCACCATCTTCAATc < 1:2436555/62‑1 (MQ=255) tACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAGGTCACCATCTTCAATc < 1:2235149/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:2246182/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:982212/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:899624/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:857193/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:462154/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:3065912/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:2989954/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:2972341/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:2844567/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:281022/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:1004862/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:2107710/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:2099083/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:2063316/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:1947881/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:1724492/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:1694448/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:1676636/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:1412850/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:123443/61‑1 (MQ=255) cTGTAACTGAATGCCACGGTTC‑GGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGc < 1:1097523/61‑1 (MQ=255) | CGCTTCACGACGCGCCGCCAGTTCGGCATCGCTTACCTGTAACTGAATGCCACGGTTCGGGATGTCGATAGCGATCAGGTCACCATCTTCAATCAGGC > W3110S.gb/3681491‑3681588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |