Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,620,068	Δ1 bp	coding (555/666 nt)	marC ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,620,066	0	C	.	100.0%	42.6 / NA	11	coding (557/666 nt)	marC	predicted transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (9/2);  total (9/2)

TACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCC  >  W3110S.gb/1620013‑1620108                                                      |                                           tacatacCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTG‑CCACCAGcc                                   <  1:2482971/62‑1 (MQ=255)          gcagGAATCCCATTAGACGGGAGATCGCTTCAATGCCACCCTTG‑CCACCAGCCGCATAATTg                          >  1:247083/1‑62 (MQ=255)          gcagGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTG‑CCACCAGCCGCATAATTg                          >  1:1550328/1‑62 (MQ=255)          gcagGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTG‑CCACCAGCCGCATAATTg                          >  1:2271456/1‑62 (MQ=255)          gcagGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTG‑CCACCAGCCGCATAATTg                          >  1:2533404/1‑62 (MQ=255)          gcagGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTG‑CCACCAGCCGCATAATTg                          >  1:2834605/1‑62 (MQ=255)          gcagGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTG‑CCACCAGCCGCATAATTg                          >  1:2849815/1‑62 (MQ=255)          gcagGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTG‑CCACCAGCCGCATAATTg                          >  1:564826/1‑62 (MQ=255)          gcagGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTG‑CCACCAGCCGCATAATTg                          >  1:57516/1‑62 (MQ=255)          gcagGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTG‑CCACCAGCCGCATAATTg                          >  1:99110/1‑62 (MQ=255)                                  tCGCTTCAATGCCACCCTTG‑CCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTcccc  <  1:758228/62‑1 (MQ=255)                                                      |                                           TACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCC  >  W3110S.gb/1620013‑1620108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑