Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,378,662	+G	intergenic (‑156/+63)	rplM ← / ← yhcM	50S ribosomal subunit protein L13/conserved hypothetical protein with nucleoside triphosphate hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,378,658	1	.	G	75.0%	27.3 / 5.5	12	intergenic (‑152/+67)	rplM/yhcM	50S ribosomal subunit protein L13/conserved hypothetical protein with nucleoside triphosphate hydrolase domain
Reads supporting (aligned to +/- strand):  ref base . (3/0);  new base G (9/0);  total (12/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGT‑GGGGTCGCAGGATTATAGAGAAGT  >  W3110S.gb/3378600‑3378682                                                            |                         cTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTTGGAAAAAAACTTTCTTGTAACGT‑ggg                       <  1:1020024/62‑1 (MQ=255) cTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGT‑ggg                       <  1:1677553/62‑1 (MQ=255) cTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGT‑ggg                       <  1:2090809/62‑1 (MQ=255) cTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGT‑ggg                       <  1:3059710/62‑1 (MQ=255)                gCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGT‑GGGGTCGCAGGATta           >  1:2240781/1‑59 (MQ=255)                gCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGT‑GGGGTCGCAGGATta           >  1:2884523/1‑59 (MQ=255)                gCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGT‑GGGGTCGCAGGATta           >  1:370813/1‑59 (MQ=255)                       cacaAAAAGTTTTGGTAAAAAAACTTTCTTGTAACGTGGGGGTCGCAGGATTATAGAGAAGt  >  1:2039945/1‑62 (MQ=255)                       cacaAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGGTCGCAGGATTATAGAGAAGt  >  1:1221812/1‑62 (MQ=255)                       cacaAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGGTCGCAGGATTATAGAGAAGt  >  1:1281098/1‑62 (MQ=255)                       cacaAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGGTCGCAGGATTATAGAGAAGt  >  1:155161/1‑62 (MQ=255)                       cacaAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGGTCGCAGGATTATAGAGAAGt  >  1:2051267/1‑62 (MQ=255)                       cacaAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGGTCGCAGGATTATAGAGAAGt  >  1:238477/1‑62 (MQ=255)                       cacaAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGGTCGCAGGATTATAGAGAAGt  >  1:3003975/1‑62 (MQ=255)                       cacaAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGGTCGCAGGATTATAGAGAAGt  >  1:333067/1‑62 (MQ=255)                       cacaAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGGTCGCAGGATTATAGAGAAGt  >  1:409389/1‑62 (MQ=255)                                                            |                         CTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGT‑GGGGTCGCAGGATTATAGAGAAGT  >  W3110S.gb/3378600‑3378682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑