| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,638,024 | (TTG)2→3 | coding (675/2163 nt) | uvrD ← | DNA‑dependent ATPase I and helicase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,638,016 | 1 | . | T | 76.5% | 37.1 / 10.1 | 17 | coding (683/2163 nt) | uvrD | DNA‑dependent ATPase I and helicase II |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base T (13/0); total (14/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.88e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | W3110S.gb | 3,638,016 | 2 | . | G | 76.5% | 43.0 / 8.6 | 17 | coding (683/2163 nt) | uvrD | DNA‑dependent ATPase I and helicase II |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base G (13/0); total (14/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.88e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | W3110S.gb | 3,638,016 | 3 | . | T | 76.5% | 37.1 / 10.1 | 17 | coding (683/2163 nt) | uvrD | DNA‑dependent ATPase I and helicase II |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base T (13/0); total (14/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.88e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
CCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAA‑‑‑TGTTGTTGGTATCCTGGAATTCGTCCACCAGGATATTGGTAAAACGTTCGCGGTAGTG > W3110S.gb/3637959‑3638074 ||| ccGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAA‑‑‑tgtt > 1:116064/1‑62 (MQ=255)ccGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAA‑‑‑tgtt > 1:190329/1‑62 (MQ=255)ccGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAA‑‑‑tgtt > 1:2136580/1‑62 (MQ=255)ccGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAA‑‑‑tgtt > 1:2271070/1‑62 (MQ=255)ccGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAA‑‑‑tgtt > 1:360810/1‑62 (MQ=255)ccGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAA‑‑‑tgtt > 1:320214/1‑62 (MQ=255) acgaTCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAA‑‑‑tgttgt > 1:2793546/1‑61 (MQ=255) acgaTCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAA‑‑‑Tgttgtt > 1:1149360/1‑62 (MQ=255) cGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAA‑‑‑TGTTGTTGGTATCCTGGAATTc < 1:38277/61‑1 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGTTGGTATCCTGGAATTc > 1:662460/1‑61 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGTTGGTATCCTGGAATTCg > 1:977433/1‑62 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGTTGGTATCCTGGAATTCg > 1:1114678/1‑62 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGTTGGTATCCTGGAATTCg > 1:8856/1‑62 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGTTGGTATCCTGGAATTCg > 1:639246/1‑62 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGTTGGTATCCTGGAATTCg > 1:489321/1‑62 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGTTGGTATCCTGGAATTCg > 1:3084200/1‑62 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGTTGGTATCCTGGAATTCg > 1:2790188/1‑62 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGTTGGTATCCTGGAATTCg > 1:2176959/1‑62 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGTTGGTATCCTGGAATTCg > 1:2140785/1‑62 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGTTGGTATCCTGGAATTCg > 1:1980365/1‑62 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGTTGGTATCCTGGAATTCg > 1:1938074/1‑62 (MQ=255) tgtCGCCCGCCAGCAGGCGGATCCACGCGTACTGAATGTTGTTGTTGGTATCCTGGAATTCg > 1:1557220/1‑62 (MQ=255) gAA‑‑‑TGTTGTTGGTATCCTGGAATTCGTCCACCAGGATATTGGTAAAACGTTCGCGGTAgtg < 1:3060657/61‑1 (MQ=255) gAA‑‑‑TGTTGTTGGTATCCTGGAATTCGTCCACCAGGATATTGGTAAAACGTTCGCGGTAgtg < 1:1755507/61‑1 (MQ=255) ||| CCGACGATCATCACTTTGCCGGTGTCGCCCGCCAGCAGGCGGATCCACGCGTACTGAA‑‑‑TGTTGTTGGTATCCTGGAATTCGTCCACCAGGATATTGGTAAAACGTTCGCGGTAGTG > W3110S.gb/3637959‑3638074 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |