| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,923,382 | (GA)3→4 | coding (489/660 nt) | yiaD ← | predicted outer membrane lipoprotien |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,923,376 | 1 | . | G | 100.0% | 15.6 / NA | 6 | S165? (TCC→TCN) | yiaD | predicted outer membrane lipoprotien |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (6/0); total (6/0) | |||||||||||
| * | W3110S.gb | 3,923,376 | 2 | . | A | 100.0% | 13.5 / NA | 6 | S165S (TCC→TCT) | yiaD | predicted outer membrane lipoprotien |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (6/0); total (6/0) | |||||||||||
CGCTGGCAACGGAATCCGCACGTTGCTGG‑‑GAGAGACGCATGTTCAGGTCGTGACCACCCG > W3110S.gb/3923348‑3923407 || cgcTGGCAACGGAATCCGCACGTTGCTGGGAGAGAGACGCATGTTCAGGTCGTGACCAccc > 1:1696365/1‑61 (MQ=255)cgcTGGCAACGGAATCCGCACGTTGCTGGGAGAGAGACGCATGTTCAGGTCGTGACCAccc > 1:1905236/1‑61 (MQ=255)cgcTGGCAACGGAATCCGCACGTTGCTGGGAGAGAGACGCATGTTCAGGTCGTGACCAccc > 1:746731/1‑61 (MQ=255)cgcTGGCAACGGAATCCGCACGTTGCTGGGAGAGAGACGCATGTTCAGGTCGTGACCACCCg > 1:2186221/1‑62 (MQ=255)cgcTGGCAACGGAATCCGCACGTTGCTGGGAGAGAGACGCATGTTCAGGTCGTGACCACCCg > 1:2442417/1‑62 (MQ=255)cgcTGGCAACGGAATCCGCACGTTGCTGGGAGAGAGACGCATGTTCAGGTCGTGACCACCCg > 1:780518/1‑62 (MQ=255) || CGCTGGCAACGGAATCCGCACGTTGCTGG‑‑GAGAGACGCATGTTCAGGTCGTGACCACCCG > W3110S.gb/3923348‑3923407 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |